Association of creatin kinase B and peroxiredoxin 2 expression with age and embryo quality in cumulus cells
- 172 Downloads
The purpose of this study was to identify age-related oocyte or embryo markers suitable for non-invasive analysis, as women over 38 years of age experience diminished pregnancy and ovulation rates.
We used real-time quantitative PCR to examine the gene expression profiles in cumulus cells acquired from older and younger age groups. We selected 11 genes involved in three functions that directly affect cellular aging: cell cycle control, apoptosis, and metabolism.
CKB and PRDX2 were up-regulated in women older than 38 years, and the expression of these genes in cumulus cells was associated with embryo quality. In good-quality embryos, CKB expression was higher in the cumulus cells acquired from both older and younger age groups than in poor-quality embryos.
These potential relationships among cumulus cell gene expression, oocyte quality, and age may expand our understanding of oogenesis and embryo development. CKB and PRDX2 may serve as biomarkers or therapeutic targets for the developmental potential of oocytes.
KeywordsAging Creatin kinase B (CKB) Cumulus cells Embryo quality Peroxiredoxin 2 (PRDX2)
We thank Chung-I Chen, Chiu-Ping Chen, Hsiu-Hui Chen, and Ming-Chou Hung for their assistance in laboratory techniques. This study was supported by a research grant from National Science Council, Taiwan (NSC 93-2314-B -039-017 and NSC 97-2314-B-040-018) to Maw-Sheng Lee.
This study was supported by a research grant from National Science Council, Taiwan (NSC 93-2314-B-039-017 and NSC 97-2314-B-040-018) to Maw-Sheng Lee.
- 8.Cecconi S, Tatone C, Buccione R, Mangia F, Colonna R. Granulosa cell-oocyte interactions: the phosphorylation of specific proteins in mouse oocytes at the germinal vesicle stage is dependent upon the differentiative state of companion somatic cells. J Exp Zool. 1991;258:249–54.CrossRefPubMedGoogle Scholar
- 11.Vanderhyden BC, Macdonald EA, Nagyova E, Dhawan A. Evaluation of members of the TGF beta superfamily as candidates for the oocyte factors that control mouse cumulus expansion and steroidogenesis. Reprod. 2003;61:55–70.Google Scholar
- 16.Bassil S, Wyns C, Toussaint-Demylle D, Abdelnour W, Donnez J. Predictive factors of multiple pregnancy in in vitro fertilization. J Reprode Med. 1997;42:761–6.Google Scholar
- 24.Yoshida S, Yashar BM, Hiriyanna S, Swaroop A. Microarray analysis of gene expression in the aging human retina. Invest Ophthalmol Vis Sci. 2002;4:2554–60.Google Scholar
- 40.van Montfoort APA, Geraedts JPM, Dumoulin JCM, Stassen APM, Johannes LH, Evers JLH, et al. Differential gene expression in cumulus cells as a prognostic indicator of embryo viability: a microarray analysis. MHR-Basic Science of Reproductive Medicine. 2008;14:157–68.Google Scholar
- 41.Adriaenssens T, Wathlet S, Segers I, Verheyen G, De Vos A, Van der Elst J, et al. Cumulus cell gene expression is associated with oocyte developmental quality and influenced by patient and treatment characteristics. Hum Reprod. 2010;1:1–12.Google Scholar