Association of creatin kinase B and peroxiredoxin 2 expression with age and embryo quality in cumulus cells
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The purpose of this study was to identify age-related oocyte or embryo markers suitable for non-invasive analysis, as women over 38 years of age experience diminished pregnancy and ovulation rates.
We used real-time quantitative PCR to examine the gene expression profiles in cumulus cells acquired from older and younger age groups. We selected 11 genes involved in three functions that directly affect cellular aging: cell cycle control, apoptosis, and metabolism.
CKB and PRDX2 were up-regulated in women older than 38 years, and the expression of these genes in cumulus cells was associated with embryo quality. In good-quality embryos, CKB expression was higher in the cumulus cells acquired from both older and younger age groups than in poor-quality embryos.
These potential relationships among cumulus cell gene expression, oocyte quality, and age may expand our understanding of oogenesis and embryo development. CKB and PRDX2 may serve as biomarkers or therapeutic targets for the developmental potential of oocytes.
KeywordsAging Creatin kinase B (CKB) Cumulus cells Embryo quality Peroxiredoxin 2 (PRDX2)
We thank Chung-I Chen, Chiu-Ping Chen, Hsiu-Hui Chen, and Ming-Chou Hung for their assistance in laboratory techniques. This study was supported by a research grant from National Science Council, Taiwan (NSC 93-2314-B -039-017 and NSC 97-2314-B-040-018) to Maw-Sheng Lee.
This study was supported by a research grant from National Science Council, Taiwan (NSC 93-2314-B-039-017 and NSC 97-2314-B-040-018) to Maw-Sheng Lee.
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