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Extended in vitro maturation of immature oocytes from stimulated cycles: an analysis of fertilization potential, embryo development, and reproductive outcomes

  • Assisted reproduction
  • Published:
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Abstract

Purpose

To investigate 24 h in vitro maturation (IVM) of cumulus-stripped immature oocytes from stimulated cycles.

Methods

263 oocytes identified as immature after cumulus stripping for ICSI were subjected to in vitro maturation (IVM). Fertilization rates and reproductive outcomes of matured oocytes were compared against 234 in vivo matured sibling oocytes (IVO-MII-Sib) from the same cycles (n = 41). Day 2 embryo development was compared against 116 embryos from ICSI cycles having no IVM (IVO-Ext controls).

Results

While fertilization rates were similar between IVM and IVO-MII-Sib oocytes (62.1% vs. 64.0%, p = 0.9909), day 2 embryo quality was reduced in the IVM group compared with IVO-Ext controls as evidenced by fewer embryos having 4 cells (28.3% vs. 54.3%, p = 0.0026), low fragmentation (30.0% vs. 65.2%, p < 0.0001) or perfectly symmetric blastomeres (28.6% vs. 46.9%, p = 0.0371). 0 of 17 IVM embryos with known fate implanted.

Conclusion

Efficacy of 24 h IVM for cumulus-stripped GV and MI oocytes for either clinical use or study of normal meiotic maturation is questionable.

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Author information

Authors and Affiliations

  1. Department of Obstetrics, Gynecology and Reproductive Biology, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA, USA

    David E. Reichman, Joseph Politch, Elizabeth S. Ginsburg & Catherine Racowsky

  2. Division of Reproductive Biology, Department of Obstetrics and Gynecology, Boston University School of Medicine, Boston, MA, USA

    Joseph Politch

  3. Brigham & Women’s Hospital, 75 Francis Street, ASB 1+3, Rm 082, Boston, MA, 02115, USA

    Catherine Racowsky

Authors
  1. David E. Reichman
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  2. Joseph Politch
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  3. Elizabeth S. Ginsburg
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  4. Catherine Racowsky
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Corresponding author

Correspondence to Catherine Racowsky.

Additional information

Capsule Twenty-four hour in vitro maturation of immature cumulus-stripped oocytes from stimulated cycles results in embryos with compromised development and reduced implantation potential.

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Reichman, D.E., Politch, J., Ginsburg, E.S. et al. Extended in vitro maturation of immature oocytes from stimulated cycles: an analysis of fertilization potential, embryo development, and reproductive outcomes. J Assist Reprod Genet 27, 347–356 (2010). https://doi.org/10.1007/s10815-010-9416-5

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  • DOI: https://doi.org/10.1007/s10815-010-9416-5

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