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Mouse oocyte vitrification: the effects of two methods on maturing germinal vesicle breakdown oocytes

  • Fertility Preservation
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Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Evaluation of viability and subsequent developmental ability of mouse germinal vesicle breakdown oocytes vitrified in conventional straws.

Methods

Oocytes with compact cumulus cells were cultured for 3 h in TCM199 medium GVBD and vitrified by two methods: the step-wise and single-step. After vitrification, the oocytes were thawed, and subjected to in vitro maturation and in vitro fertilization. Oocyte survival (post-thaw) was assessed by morphological appearance and staining, using propidium iodide (PI)/Hoechst 33342. The oocyte maturation and fertilization rates were examined in vitro.

Results

In the single-step method the rates of post thaw survival, maturation to metaphase II and cleavage (2-cell embryos) were 58.68%, 56.41% and 38.63%, respectively. In the step-wise method, the corresponding rates were 81.75%, 68.59% and 51.80%, respectively.

Conclusion

Vitrification of mouse germinal vesicle breakdown oocytes by the step-wise method had the advantage of maintaining the viability and subsequent production of 2-cell embryos. In comparison with that in unvitrified control oocytes, the development of MII oocytes to 2-cell embryos was impaired following vitrification.

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Acknowledgement

This project financially supported by research deputy of Tehran University of Medical Sciences and Health services grant No. 85-04-30-4669. So, we would like to thank for their cooperation.

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Authors and Affiliations

  1. Department of Anat., Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Somayeh Khosravi-Farsani, Aligholi Sobhani & Fardin Amidi

  2. Dept. of Anat., Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran

    Reza Mahmoudi

Authors
  1. Somayeh Khosravi-Farsani
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  2. Aligholi Sobhani
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  3. Fardin Amidi
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  4. Reza Mahmoudi
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Correspondence to Reza Mahmoudi.

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Capsule

Vitrification of mouse cumulus germinal vesicle breakdown oocyte complexes by a step-wise manner had the advantage of maintaining the viability and subsequent production of 2-cell embryos.

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Khosravi-Farsani, S., Sobhani, A., Amidi, F. et al. Mouse oocyte vitrification: the effects of two methods on maturing germinal vesicle breakdown oocytes. J Assist Reprod Genet 27, 233–238 (2010). https://doi.org/10.1007/s10815-010-9411-x

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  • DOI: https://doi.org/10.1007/s10815-010-9411-x

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