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Journal of Assisted Reproduction and Genetics

, Volume 27, Issue 2–3, pp 111–120 | Cite as

Ontological evaluation of transcriptional differences between sperm of infertile males and fertile donors using microarray analysis

  • Sandra García-Herrero
  • Nicolás Garrido
  • José Antonio Martínez-Conejero
  • José Remohí
  • Antonio Pellicer
  • Marcos Meseguer
Gamete Biology

Abstract

Purpose

To catalogue Gene Ontology terms in the sperm of infertile human males vs. donors of proven fertility by analyzing five samples from each of the two groups (five aliquots from fresh sperm and five post-swim-up).

Methods

Microarray technology was employed to study the mRNA profile of both fresh and post-swim-up pooled samples from infertile males and donors.

Results

Genes that were differentially expressed in the two populations and expressed in only one of two were analyzed to determine the gene products in terms of their associated Gene Ontology terms. Each group presented a different number and pattern of Gene Ontology terms.

Conclusions

We found differences in Gene Ontology terms between the two groups. These differences could potentially be employed to establish markers of fertility success and to identify cellular processes and complex systems related with male infertility.

Keywords

DAVID bioinformatics Gene Ontology Microarray and sperm 

Notes

Acknowledgements

The authors would like to express their gratitude to technical personnel of the Andrology and IVF-Sperm Laboratory for their cooperation in this study.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Sandra García-Herrero
    • 1
    • 2
  • Nicolás Garrido
    • 1
  • José Antonio Martínez-Conejero
    • 1
  • José Remohí
    • 1
  • Antonio Pellicer
    • 1
  • Marcos Meseguer
    • 1
  1. 1.Instituto Universitario IVI ValenciaUniversidad de ValenciaValenciaSpain
  2. 2.Andrology LaboratoryInstituto Universitario IVI ValenciaValenciaSpain

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