Journal of Assisted Reproduction and Genetics

, Volume 27, Issue 1, pp 13–16 | Cite as

Efficiency of hyaluronic acid (HA) sperm selection

  • Lodovico Parmegiani
  • Graciela Estela Cognigni
  • Walter Ciampaglia
  • Patrizia Pocognoli
  • Francesca Marchi
  • Marco Filicori



Hyaluronic Acid (HA) has a role as “physiologic selector” for spermatozoa prior to intracytoplasmic sperm injection (ICSI). The objective of this study is to analyze the results achievable by the introduction of a routine HA-ICSI programme.


We retrospectively observed 293 couples treated with HA-ICSI versus 86 couples treated with conventional PVP-ICSI (historical control group). ICSI was performed on a limited number of oocytes per patient (1–3) according to Italian IVF law at the time of the study. Main outcome measures observed were: fertilization, embryo quality, implantation and pregnancy.


This study showed that Injection of HA-bound spermatozoa (HA-ICSI) significantly improves embryo quality and implantation.


If wider multi-center randomized studies will confirm these beneficial effects on ICSI outcome, HA could be considered as a routine choice for “physiologic” sperm selection prior to ICSI.


Hyaluronic Acid selection HA-ICSI ICSI Physiologic ICSI Sperm selection 


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Lodovico Parmegiani
    • 1
  • Graciela Estela Cognigni
    • 1
  • Walter Ciampaglia
    • 1
  • Patrizia Pocognoli
    • 1
  • Francesca Marchi
    • 2
  • Marco Filicori
    • 1
  1. 1.Reproductive Medicine Unit—GynePro Medical CentersBolognaItaly
  2. 2.GynePro Medical CentersArcoItaly

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