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Unexpected detrimental effect of Insulin like growth factor-1 on bovine oocyte developmental competence under heat stress

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Abstract

Purpose

To investigate the effects of Insulin like growth factor-1 (IGF-1) on bovine oocyte developmental competence under heat stress.

Methods

In Experiment 1, bovine cumulus-oocyte complexes (COCs) were cultured at 38.5 or 41°C for the first 12 h of maturation in the presence of either 100 ng/ml human recombinant (hr)-IGF-1 or acetic acid. In Experiment 2, COCs were cultured in 38.5 or 41°C for the first 12 h of maturation in the presence either of 100 ng/ml hr-IGF-1 or acetic acid. After fertilization, putative zygotes were cultured for 8 days.

Results

In experiment 1, addition of rh-IGF-1 to maturation medium at 38.5°C significantly increased the proportion of M II oocytes and decreased the percentage of TUNEL-positive oocytes compared to the other groups. However, addition of rh-IGF-1 to maturation medium under heat stress increased the percentage of TUNEL-positive oocytes. In experiment 2, addition of rh-IGF-1 under heat sress did not affect cleavage rate, whereas, blastocyst formation rate decreased in heat-stressed and heat-stressed plus rh-IGF-1 groups. Similarly, The number of trophectoderm cells and total cell number were decreased in heat-stressed and heat-stressed plus rh-IGF-1 groups and the percentage of TUNEL-positive nuclei were increased in heat-stressed and heat-stressed plus rh-IGF-1 groups compared to the other groups.

Conclusion

The results of the present study demonstrate that IGF-1 decreases oocyte developmental competence and total cell number and increases TUNEL-positive nuclei at heat stress condition. These unexpected results of IGF-1 during maturation period under heat stress condition warrant further optimizations and investigations.

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Acknowledgments

This study was funded by Royan institute of Iran, grant number 86/31684. The authors would like to thank University of Tehran and M. Hajian and L. Hosseini from the laboratory of the Royan institute, Isfahan campus for their technical supports.

Author information

Authors and Affiliations

  1. Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, P.O.Box#4111, Karaj, Iran

    Mahdi Zhandi, Armin Towhidi & Ahmad Zare-Shahneh

  2. Department of Clinical and Experimental Embryology, Cell Sciences Research Centre, Royan Institute, Tehran, Iran

    Mohammad Hossein Nasr-Esfahani & Poopak Eftekhari-Yazdi

Authors
  1. Mahdi Zhandi
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  2. Armin Towhidi
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  3. Mohammad Hossein Nasr-Esfahani
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Correspondence to Mahdi Zhandi.

Additional information

Capsule Bovine oocyte developmental competance is decreased by simultaneously effect of IGF-1 and heat stress.

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Zhandi, M., Towhidi, A., Nasr-Esfahani, M.H. et al. Unexpected detrimental effect of Insulin like growth factor-1 on bovine oocyte developmental competence under heat stress. J Assist Reprod Genet 26, 605–611 (2009). https://doi.org/10.1007/s10815-009-9364-0

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  • DOI: https://doi.org/10.1007/s10815-009-9364-0

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