Top quality embryos at day 2: a prerequisite for single blastocyst transfer? An observational cohort study in women under 36

  • Fabrice GuerifEmail author
  • Malak Lemseffer
  • Milène Blanchard
  • Dominique Royere
Assisted Reproduction



While extended culture has been considerably improved, some questions remain regarding the application of Single Blastocyst Transfer (SBT).


An observational cohort study was undertaken with 456 women under 36 years old and assigned to SBT on a voluntary basis. The main outcome was the cumulative delivery rate per couple according to the number of Top Quality Embryos (TQE) on day 2 (Group 1= ≥2 TQE, Group 2= 1 TQE and Group 3= 0 TQE).


Rate of transfer and mean number of frozen blastocyts were higher in Group 1 compared to Group 3. As a consequence, the cumulative delivery rate per couple was higher in Group 1 (47.9%) compared to Group 3 (34.9%).


Single blastocyst transfer combining fresh and frozen cycles, might be a worthwhile strategy irrespective of embryo quality on day 2 providing good delivery rates while keeping the rate of multiple deliveries low.


Cumulative delivery Multiple delivery Single blastocyst transfer Top quality embryo 


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Fabrice Guerif
    • 1
    • 2
    • 3
    Email author
  • Malak Lemseffer
    • 1
    • 2
    • 3
  • Milène Blanchard
    • 1
    • 2
    • 3
  • Dominique Royere
    • 1
    • 2
    • 3
  1. 1.Service de Médecine et Biologie de la ReproductionCHRU BretonneauToursFrance
  2. 2.Université François RabelaisToursFrance
  3. 3.INRA UMR 85 - CNRS UMR 6175NouzillyFrance

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