Journal of Assisted Reproduction and Genetics

, Volume 26, Issue 8, pp 451–453 | Cite as

Birth of a healthy male infant after transfer of vitrified-warmed blastocysts derived from intracytoplasmic sperm injection with vitrified-warmed oocytes and frozen-thawed spermatozoa

  • Koichi KyonoEmail author
  • Yukiko Nakajo
  • Masakazu Doshida
  • Mayumi Toya
  • Yasuhisa Araki



To report a successful delivery of a healthy baby after transfer of vitrified-warmed blastocysts derived from introcytoplasmic sperm injection (ICSI) with vitrified-warmed oocytes and frozen-thawed sperm.


A female patient and her husband with non-obstructive azoospermia received a transfer of vitrified-warmed blastocysts from vitrified-warmed oocytes and frozen-thawed sperm. The main outcome measures were fertilization, pregnancy and birth.


Nine oocytes were matured and vitrified. When the vitrified oocytes were warmed, six survived with good quality morphology. Using ICSI, frozen-thawed sperm was injected into the six warmed oocytes that survived, and the fertilization rate was 100%. The zygotes were cultured, and five of six early embryos became blastocysts. One of them was transferred, but pregnancy was not achieved. The second time around, two vitrified-warmed blastocysts were transferred resulting in pregnancy, and a healthy boy was delivered.


This is a rare case of a successful birth using a vitrified-warmed blastocyst grown after ICSI with a vitrified-warmed oocyte and frozen-thawed sperm.


Frozen-thawed sperm ICSI Vitrified-warmed blastocysts Vitrified-warmed oocytes 


Financial support



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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Koichi Kyono
    • 1
    Email author
  • Yukiko Nakajo
    • 1
  • Masakazu Doshida
    • 1
  • Mayumi Toya
    • 1
  • Yasuhisa Araki
    • 2
  1. 1.Kyono ART ClinicMitsui-SeimeiSendaiJapan
  2. 2.The Institute for ARMTSetagunJapan

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