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Journal of Assisted Reproduction and Genetics

, Volume 26, Issue 8, pp 451–453 | Cite as

Birth of a healthy male infant after transfer of vitrified-warmed blastocysts derived from intracytoplasmic sperm injection with vitrified-warmed oocytes and frozen-thawed spermatozoa

  • Koichi Kyono
  • Yukiko Nakajo
  • Masakazu Doshida
  • Mayumi Toya
  • Yasuhisa Araki
CRYOPRESERVATION

Abstract

Purpose

To report a successful delivery of a healthy baby after transfer of vitrified-warmed blastocysts derived from introcytoplasmic sperm injection (ICSI) with vitrified-warmed oocytes and frozen-thawed sperm.

Methods

A female patient and her husband with non-obstructive azoospermia received a transfer of vitrified-warmed blastocysts from vitrified-warmed oocytes and frozen-thawed sperm. The main outcome measures were fertilization, pregnancy and birth.

Results

Nine oocytes were matured and vitrified. When the vitrified oocytes were warmed, six survived with good quality morphology. Using ICSI, frozen-thawed sperm was injected into the six warmed oocytes that survived, and the fertilization rate was 100%. The zygotes were cultured, and five of six early embryos became blastocysts. One of them was transferred, but pregnancy was not achieved. The second time around, two vitrified-warmed blastocysts were transferred resulting in pregnancy, and a healthy boy was delivered.

Conclusions

This is a rare case of a successful birth using a vitrified-warmed blastocyst grown after ICSI with a vitrified-warmed oocyte and frozen-thawed sperm.

Keywords

Frozen-thawed sperm ICSI Vitrified-warmed blastocysts Vitrified-warmed oocytes 

Notes

Financial support

None.

References

  1. 1.
    Chen C. Pregnancy after human oocyte cryopreservation. Lancet. 1986;1:884–6.CrossRefPubMedGoogle Scholar
  2. 2.
    Katayama KP, Stehlik J, Kuwayama M, Kato O, Stehlik E. High survival rate of vitrified human oocytes results in clinical pregnancy. Fertil Steril. 2003;80:223–4.CrossRefPubMedGoogle Scholar
  3. 3.
    Kyono K, Fuchinone K, Yagi A, Nakajo Y, Yamashita A, Kumagai S. Successful pregnancy and delivery after transfer of a single blastocyst derived from a vitrified mature human oocyte. Fertil Steri. 2005;84:1017e5–6.Google Scholar
  4. 4.
    Kuwayama M, Vajta G, Kato O, Leibo SP. Highly efficient vitrification method for cryopreservation of human oocytes. Reproductive BioMedicine Online. 2005;11:300–308.PubMedGoogle Scholar
  5. 5.
    Yang D, Brown SE, Nguyen K, Reddy V, Brubaker C, Winslow KL. Live birth after the transfer of human embryos developed from cryopreserved oocytes harvested before cancer treatment. Fertil Steril. 2007;87:e1–4.CrossRefPubMedGoogle Scholar
  6. 6.
    Porcu E, Venturoli S, Damiano G, Ciotti PM, Notarangelo L, Paradisi R, et al. Live birth after the transfer of human embryos developed from cryopreserved oocytes harvested before cancer treatment. Reproductive BioMedicine Online. 2008;17:267–269.Google Scholar
  7. 7.
    Lassalle B, Testart J, Renard JP. Human embryo features that influence the success of cryopreservation with the use of 1,2 propanediol. Fertil Steril. 1985;44:645–51.PubMedGoogle Scholar
  8. 8.
    Borini A, Bonu MA, Coticchio G, Cattoli M, Bianchi V, Cattoli M, et al. Pregnancies and birth after oocyte cryopreservation. Fertil Steril. 2004;82:601–605.CrossRefPubMedGoogle Scholar
  9. 9.
    Levi Setti PE, Albani E, Novara PV, Cesana A, Morreale G. Cryopreservation of supernumerary oocytes in IVF/ICSI cycles. Hum Reprod. 2006;22:370–375.Google Scholar
  10. 10.
    Debra A, Gook Lyndon Hale, Edgar David H. Live birth following transfer of a cryopreserved embryo generated from a cryopreserved oocyte and cryopreserved sperm: Case report. J Assist Reprod Genet. 2007;24:43–45.CrossRefGoogle Scholar
  11. 11.
    Greco E, Iacobelli M, Rienzi L, Fabris MGF, Tesoyio N, Tesarik J. Birth of a healthy boy after cryopreserved oocyte with cryopreserved testicular spermatozoa from a man with nonmasaic Klinefelter syndrome. Fertil Steril. 2008;89:991–e5–7.CrossRefPubMedGoogle Scholar
  12. 12.
    Boldt J, Tidswell N, Sayers A, Kilani R, Cline D. Human oocyte cryopreservation: 5-year experience with a sodium-depleted slow freezing method. Reproductive BioMedicine Online. 2006;13:96–100.PubMedCrossRefGoogle Scholar
  13. 13.
    Coticchio G, Santis LD, Rossi G, Borini A, Albertini D, Scaravelli G, et al. Sucrose concentration influences the rate of human oocytes with normal spindle and chromosome configurations after slow-cooling cryopreservation. Hum Reprod. 2006;21:1771–6.CrossRefPubMedGoogle Scholar
  14. 14.
    Setti PEL, Albani E, Novara PV, Cesana A, Bianchi S, Negri L. Normal birth after transfer of cryopreserved human embryos generated by microinjection of cryopreserved testicular spermatozoa into cryopreserved human oocytes. Fertil Steril. 2005;83:1041–e9–10.Google Scholar
  15. 15.
    Chen GA, Cai XY, Lian Y, Zheng XY, Qiao J, Chen XN, et al. Normal birth from cryopreserved embryos after intracytoplasmic sperm injection of frozen semen into vitrified human oocytes. Hum Fertil (Camb). 2008 Mar;11(1):49–51.CrossRefGoogle Scholar
  16. 16.
    Montag M, Van der Ven K, Dorn C, Isachenko V, Isachenko E, van der Ven. Birth after double cryopreservation of human oocytes at metaphase II and pronuclear stages. Fertil Steril. 2006;85:751–e5–7.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Koichi Kyono
    • 1
  • Yukiko Nakajo
    • 1
  • Masakazu Doshida
    • 1
  • Mayumi Toya
    • 1
  • Yasuhisa Araki
    • 2
  1. 1.Kyono ART ClinicMitsui-SeimeiSendaiJapan
  2. 2.The Institute for ARMTSetagunJapan

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