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Achieving high survival rate following cryopreservation after isolation of prepubertal mouse spermatogonial cells

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Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Isolating spermatogonia cells with high purity and viability and achieving better survival rate following cryopreservation

Methods

Isolating the cells by Magnetic Activating Cell Sorting (MACS) method using anti CD49f (α6 integrin) antibody and Dynabeads and freezing in DMSO-based freezing mediums containing three different FBS concentrations of 50%, 60% and 70%.

Results

The mean (±SD) purity of the isolated cells was 92.52 ± 3.57 (range 92.43–98.25). The cells frozen in group I, II and III had mean 39.60 ± 1.48 (range 37.98–41.62), 89.05 ± 3.83 (range 80.83–90.33) and 90.52 ± 1.71 (range 89.07–92.52) viability, respectively.

Conclusion

Higher viable cell counts and purity can be attained by the use of α6 integrin and magnetic beads. After the thawing of spermatogonial cells, optimum viability was achieved in freezing media containing 60% FBS.

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Acknowledgment

The author would like to thank Dr Reza Omani Samani, Dr. Daneshzadeh, Dr. Khakzad, Miss Shabani and Dr. Afshani for their creative ideas and practical help and support during the time of study.

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Authors and Affiliations

  1. Royan Institute,Andrology Lab, P.O.Box: 19395-4644, Tehran, Iran

    Firooz Jannat Alipoor, Mohammad Ali Sadighi Gilani, Poopak Eftekhari-Yazdi, Ali Daliri Hampa, Hani Hosseinifar, Hiva Alipour & Mehdi Lotfi Panah

  2. Faculty of Science, School of Biology, Tehran University, Tehran, Iran

    Firooz Jannat Alipoor & Hani Hosseinifar

Authors
  1. Firooz Jannat Alipoor
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  2. Mohammad Ali Sadighi Gilani
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Correspondence to Firooz Jannat Alipoor.

Additional information

Capsule In this study spermatogonia cells from mouse testis were isolated by MACS method and the effect of FBS concentration on cell survival was assessed during cryopreservation.

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Alipoor, F.J., Gilani, M.A.S., Eftekhari-Yazdi, P. et al. Achieving high survival rate following cryopreservation after isolation of prepubertal mouse spermatogonial cells. J Assist Reprod Genet 26, 143–149 (2009). https://doi.org/10.1007/s10815-009-9298-6

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  • DOI: https://doi.org/10.1007/s10815-009-9298-6

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