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Improvement of embryonic development and production of offspring by transferring meiosis-II chromosomes of senescent mouse oocytes into cytoplasts of young mouse oocytes

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Abstract

Purpose

The effects of reciprocal transplantation of meiosis-II chromosomes between senescent and young mouse oocytes were evaluated based on pre- and post-implantation development ability of resultant embryos.

Methods

Karyoplasts including meiosis-II chromosomes of oocytes from senescent Rockefeller mouse/Ms-Rb(6, 15) females (10 to 12 months, age-related infertile mice) were transferred into cytoplasts of oocytes from young F1 females (3 to 5 months). Reconstructed oocytes were fertilized in vitro, and then the resultant embryos were cultured in vitro and transferred to recipient mice.

Results

The reconstructed oocytes that consisted of aged-karyoplasts and young-cytoplasts showed significantly improved embryonic development (from 23.2% to 30.0%) and development to term (from 6.3% to 27.1%, P < 0.05) as compared with the oocytes reconstructed from young-karyoplasts and aged-cytoplasts.

Conclusions

The present study showed successful rejuvenation for age-related infertility using transplantation of meiosis-II chromosomes in animal experimental models.

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Acknowledgement

This work was supported in part by a Grant-in-Aid for Scientific Research (No. 17580245) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan

    Akinori Mitsui, Midori Yoshizawa, Hiromichi Matsumoto & Emiko Fukui

  2. Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University, 350 Minemachi, Utsunomiya, Tochigi, 321-8505, Japan

    Akinori Mitsui, Midori Yoshizawa, Hiromichi Matsumoto & Emiko Fukui

Authors
  1. Akinori Mitsui
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  2. Midori Yoshizawa
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  3. Hiromichi Matsumoto
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  4. Emiko Fukui
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Corresponding author

Correspondence to Midori Yoshizawa.

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Capsule Transplantation of meiosis-II chromosomes of senescent mouse oocytes into cytoplasts of young mouse oocytes improves subsequent embryonic and fetal development until birth.

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Mitsui, A., Yoshizawa, M., Matsumoto, H. et al. Improvement of embryonic development and production of offspring by transferring meiosis-II chromosomes of senescent mouse oocytes into cytoplasts of young mouse oocytes. J Assist Reprod Genet 26, 35–39 (2009). https://doi.org/10.1007/s10815-008-9282-6

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  • DOI: https://doi.org/10.1007/s10815-008-9282-6

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