Journal of Assisted Reproduction and Genetics

, Volume 25, Issue 8, pp 375–381 | Cite as

Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

  • Enver Kerem Dirican
  • Osman Denizhan Özgün
  • Süleyman Akarsu
  • Kadir Okhan Akın
  • Özge Ercan
  • Mukaddes Uğurlu
  • Çağrı Çamsarı
  • Oya Kanyılmaz
  • Adnan Kaya
  • Ali Ünsal



Magnetic activated cell sorting (MACS) eliminates apoptotic spermatozoa based on the presence of externalized phosphatidylserine residues. We evaluated the outcome of male fertility treatment when intracytoplasmic sperm injection (ICSI) into human oocytes was performed with non-apoptotic MACS-selected spermatozoa.


196 couples were treated by ICSI following spermatozoa preparation by MACS (study group; 122 couples) or density gradient centrifugation (DGC) (control group; 74 couples). Fertilization, cleavage, pregnancy, and implantation rates were analyzed.


The percentage of sperm with normal morphology after MACS selection was improved. Cleavage and pregnancy rates were higher, respectively, in the study group than in control. A slightly higher implantation rate was also observed in the study group.


MACS selection of human spermatozoa increased cleavage and pregnancy rates in oligoasthenozoospermic ART cases. This novel method for selecting non-apoptotic spermatozoa for ICSI is safe and reliable, and may improve the assisted reproduction outcome.


Apoptosis Spermatozoa Intracytoplasmic sperm injection Pregnancy rate 


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Enver Kerem Dirican
    • 1
  • Osman Denizhan Özgün
    • 2
  • Süleyman Akarsu
    • 3
  • Kadir Okhan Akın
    • 4
  • Özge Ercan
    • 1
  • Mukaddes Uğurlu
    • 5
  • Çağrı Çamsarı
    • 5
  • Oya Kanyılmaz
    • 6
  • Adnan Kaya
    • 2
  • Ali Ünsal
    • 7
  1. 1.EmbryologyMAYA Center for Assisted Reproductive TechnologiesAnkaraTurkey
  2. 2.Obstetrics and GynecologyMAYA Center for Assisted Reproductive TechnologiesAnkaraTurkey
  3. 3.Obstetrics and GynecologyKeçiören Education and Research HospitalAnkaraTurkey
  4. 4.BiochemistryKeçiören Education and Research HospitalAnkaraTurkey
  5. 5.AndrologyMAYA Center for Assisted Reproductive TechnologiesAnkaraTurkey
  6. 6.Public HealthMAYA Center for Assisted Reproductive TechnologiesAnkaraTurkey
  7. 7.UrologyKeçiören Education and Research HospitalAnkaraTurkey

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