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Effect of human oviductal in vitro secretion on human sperm DNA integrity

  • Andrology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

In the female genital tract spermatozoa interact with the oviductal secretion. The aim of the study was to evaluate the effect of conditioned media (CM) from cultures of human oviductal tissue, on sperm DNA integrity. The effect of H2O2 on sperm DNA integrity, before and after incubation under capacitating conditions, was also investigated.

Methods

Motile sperm obtained from normozoospermic semen samples were incubated (4 h or 22 h) in the presence or absence of CM and further exposed to H2O2. DNA damage was detected by the comet assay.

Results

The CM significantly reduced the DNA damage associated with sperm incubation, and also decreased the effect of H2O2 after 4 h incubation, compared to controls. The H2O2 caused a dose-dependent deleterious effect on sperm DNA integrity both before and following 22 h of capacitation.

Conclusion

The oviductal tissue CM increased the stabilization of the sperm DNA structure under culture conditions.

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Acknowledgements

The present study was partially supported by grants from CONICET (PID 02153) and FONCYT (PICT 0115092).

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Correspondence to Sergio Ghersevich.

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Robert, C., Caille, A., Zumoffen, C. et al. Effect of human oviductal in vitro secretion on human sperm DNA integrity. J Assist Reprod Genet 25, 263–270 (2008). https://doi.org/10.1007/s10815-008-9228-z

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  • DOI: https://doi.org/10.1007/s10815-008-9228-z

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