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Effect of the size of zona pellucida opening by laser assisted hatching on clinical outcome of frozen cleaved embryos that were cultured to blastocyst after thawing in women with multiple implantation failures of embryo transfer: a retrospective study

  • Assisted Reproduction
  • Published:
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Abstract

Purpose

To evaluate the effect of the size of zona pellucida opening by laser assisted hatching for frozen cleaved embryo that were thawed after both fresh and frozen cleaved embryo transfer cycles failed and were cultured to blastocyst after thawing in patients with multiple implantation failures.

Materials and methods

Of 101 consecutive procedures (October 2003 to June 2006), 30 patients declined to perform assisted hatching and were selected as control group, 40 patients had 40 μm opening of the zona (October 2003 to January 2005), 31 patients had 50% of the zona opening (February 2005 to June 2006).

Results

The pregnancy, implantation and delivery rates were significantly higher in 50% opening group (74%, 52% and 65%) compared to control group (17%, 10% and 13%; P < 0.01) and 40 μm opening group (43%, 27% and 38%; P < 0.04).

Conclusions

The size of the zona pellucida opening may affect the outcome of frozen cleaved embryo transfer.

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Authors and Affiliations

  1. Kinutani Women’s Clinic, 8-23-4F, Hondori, Naka-ku, Hiroshima, 730-0035, Japan

    Kenichiro Hiraoka, Megumi Fuchiwaki, Kaori Hiraoka, Tomoyo Murakami, Masayuki Kinutani & Kazuo Kinutani

  2. Graduate School of Applied Biosciences, Hiroshima Prefectural University, Hiroshima, 727-0023, Japan

    Toshitaka Horiuchi

Authors
  1. Kenichiro Hiraoka
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  2. Megumi Fuchiwaki
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  3. Kaori Hiraoka
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  4. Toshitaka Horiuchi
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  5. Tomoyo Murakami
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  6. Masayuki Kinutani
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  7. Kazuo Kinutani
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Correspondence to Kenichiro Hiraoka.

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Capsule

The size of the zona pellucida opening by laser assisted hatching may affect the outcome of frozen cleaved embryo transfer in multiple implantation failures.

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Hiraoka, K., Fuchiwaki, M., Hiraoka, K. et al. Effect of the size of zona pellucida opening by laser assisted hatching on clinical outcome of frozen cleaved embryos that were cultured to blastocyst after thawing in women with multiple implantation failures of embryo transfer: a retrospective study. J Assist Reprod Genet 25, 129–135 (2008). https://doi.org/10.1007/s10815-008-9214-5

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  • DOI: https://doi.org/10.1007/s10815-008-9214-5

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