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High developmental potential in vitro and in vivo of cattle embryos cloned without micromanipulators

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Abstract

Purpose

In order to simplify cloning, a new method that does not require micromanipulators was used. We aimed to evaluate the developmental potential of two bovine cell lines upon cloning.

Materials and methods

In vitro matured bovine oocytes, were released from zona pellucida, enucleated, fused to foetal or adult somatic donor cells. The reconstructed embryos were reprogrammed, activated and cultured until blastocyst stage. No micromanipulators were used. Blastocyst rate and quality was scored. Some expanded (d7) blastocysts were transferred to recipient cattle and collected back at d17 to assess elongation.

Results

High developmental potential in vitro of cloned embryos to expanded (d7) blastocysts was achieved (52.6%). In one cell line, 65.7% of blastocysts was scored. Most blastocysts (87.4%) were graded as excellent. In vivo development to elongation (day-17) in temporary recipient cows also showed a high developmental potential (11/18 transferred blastocysts elongated).

Conclusions

Hand-made cloning is an efficient alternative for cloning in cattle.

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Acknowledgements

Authors wish to acknowledge Dr. Gabor Vajta for introducing us to hand made cloning and for continuous and encouraging support. Drs. P. Bustamante, O. Skewes, X. Letelier for support with animals. Part of this work was supported by Grant FIA PIC-2005–1-P-097, from the Ministry of Agriculture of Chile.

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Authors and Affiliations

  1. Animal Science, University of Concepcion, Avenida Vicente Méndez 595, Chillán, 537, Chile

    Lleretny Rodríguez, Felipe I. Navarrete, Heribelt Tovar, José F. Cox & Fidel Ovidio Castro

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  1. Lleretny Rodríguez
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  2. Felipe I. Navarrete
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  3. Heribelt Tovar
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  4. José F. Cox
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  5. Fidel Ovidio Castro
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Correspondence to Fidel Ovidio Castro.

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Rodríguez, L., Navarrete, F.I., Tovar, H. et al. High developmental potential in vitro and in vivo of cattle embryos cloned without micromanipulators. J Assist Reprod Genet 25, 13–16 (2008). https://doi.org/10.1007/s10815-007-9194-x

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  • DOI: https://doi.org/10.1007/s10815-007-9194-x

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