Journal of Assisted Reproduction and Genetics

, Volume 24, Issue 10, pp 445–449 | Cite as

Mitochondria transfer can enhance the murine embryo development

  • Yu-Chiao Yi
  • Ming-Jer Chen
  • Jason Yen-Ping Ho
  • Hwa-Fen Guu
  • Esther Shih-Chu Ho



To evaluate the effect of mitochondrial transfer on embryonic development.

Materials and methods

Mitochondria concentrates were collected from murine hepatocytes and fertilized murine zygotes from young and older mice in the 2PN stage were subjected to mitochondrial transfer and cultured in vitro to evaluate the embryonic development.


After extended in vitro culture, 37.65% and 20.91% embryos from the young mice developed to the blastocyst stage in the injected and control groups respectively, which is statistically significant. There was no difference in terms of hatching rates (1.76% and 1.82% respectively). Zygotes from the older mice (>20 weeks old) that received mitochondrial transfer also had a better developmental outcome than the control group (54.35% and 18.92% developed to morula stage, 43.48% and 8.11% developed to the blastocyst stage respectively), which is statistically significant.


Our results for the murine model provide direct scientific evidence that mitochondrial transfer improves embryonic development. However, potential risks such as mitochondrial heteroplasmy, nuclear-mitochondrial interaction and epigenetic aspects all deserve further evaluation before mitochondrial transfer is applied clinically.


Mitochondria Embryo Zygote 


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Yu-Chiao Yi
    • 1
    • 2
  • Ming-Jer Chen
    • 1
    • 3
  • Jason Yen-Ping Ho
    • 1
    • 4
  • Hwa-Fen Guu
    • 1
  • Esther Shih-Chu Ho
    • 1
    • 3
  1. 1.Department of Obstetrics and GynecologyTaichung Veterans General HospitalTaichungTaiwan
  2. 2.Institute of Biochemistry and BiotechnologyChung Shan Medical UniversityTaichungTaiwan
  3. 3.School of MedicineNational Yang Ming UniversityTaipeiTaiwan
  4. 4.Institute of Biomedical SciencesNational Chung Hsing UniversityTaichungTaiwan

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