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Sperm motility stimulation and preservation with various concentrations of follicular fluid

  • Andrology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The purpose of this study is to evaluate the optimal concentrations of follicular fluid (FF) for sperm motility stimulation and preservation.

Methods

Thirty normal semen samples and FF from women in an in vitro fertilization programme were used in an experiment. The semen was processed and incubated in Earle culture media with 20, 50, 80, and 100% FF (v/v) with controls. Sperm motility was evaluated and followed up for 48 h.

Results

FF could stimulate progressive sperm motility at all concentrations and last for at least 12 h. However, at more than 50% v/v of FF, sperm demonstrated a rapid decline in progressive motility after 12 h of incubation compared to other concentrations and the control group.

Conclusion

FF can stimulate the sperm motility properly at not more than 50% (v/v) concentration.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Faculty of Medicine, Prince of Songkhla University, HatYai, Songkla, 90110, Thailand

    Chatpavit Getpook & Suwadee Wirotkarun

Authors
  1. Chatpavit Getpook
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  2. Suwadee Wirotkarun
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Correspondence to Chatpavit Getpook.

Additional information

Follicular fluid can stimulate sperm motility optimally at not more than 50% v/v concentration.

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Getpook, C., Wirotkarun, S. Sperm motility stimulation and preservation with various concentrations of follicular fluid. J Assist Reprod Genet 24, 425–428 (2007). https://doi.org/10.1007/s10815-007-9145-6

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  • DOI: https://doi.org/10.1007/s10815-007-9145-6

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