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Analysis of meiotic segregation patterns and interchromosomal effects in sperm from six males with Robertsonian translocations

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To provide more genetic information about meiotic segregation behavior and the possibility of interchromosomal effects (ICE) in spermatozoa from carriers of Robertsonian (Rob) translocations.

Materials and methods

Meiotic segregation behavior in spermatozoa from six carriers of Rob translocations, four t(13;14), one t(14;22) and one t(13;21), was investigated by dual fluorescence in-situ hybridization (FISH). Aneuploidy for chromosomes 18, X and Y was studied by triple FISH.

Results

The rate of normal/balanced spermatozoa resulting from alternate segregation ranged from 78.14 to 86.88%. The frequency of unbalanced spermatozoa resulting from adjacent segregation varied between 11.70 and 19.53%. The higher frequencies of aneuploidy for sex chromosome were observed in three Rob translocation carriers. In addition, the increased rates of diploid were found in two t(13;14) carriers.

Conclusions

Alternate segregation is dominant in the different types of Rob translocations. Some carriers may be at an increased risk for ICE.

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Authors and Affiliations

  1. The Centre of Reproductive Medicine, Peking University Third Hospital, Beijing, 100083, China

    Yongjian Chen, Jin Huang, Ping Liu & Jie Qiao

Authors
  1. Yongjian Chen
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  2. Jin Huang
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  3. Ping Liu
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  4. Jie Qiao
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Corresponding author

Correspondence to Yongjian Chen.

Additional information

An increased aneuploidy for sex chromosome observed in three Robertsonian translocation carriers suggests that an interchromosomal effect is likely in some carriers.

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Chen, Y., Huang, J., Liu, P. et al. Analysis of meiotic segregation patterns and interchromosomal effects in sperm from six males with Robertsonian translocations. J Assist Reprod Genet 24, 406–411 (2007). https://doi.org/10.1007/s10815-007-9137-6

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  • DOI: https://doi.org/10.1007/s10815-007-9137-6

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