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The utility of serum leptin and follicular fluid leptin, estradiol, and progesterone levels during an in vitro fertilization cycle

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Abstract

Purpose: To prospectively evaluate serum and follicular fluid leptin, estradiol, and progesterone levels during in vitro fertilization.

Methods: Prospective observational study measuring serum levels at six points during the IVF cycle and follicular fluid at the time of retrieval.

Results: Serum leptin and estradiol levels both significantly increased for the individual patients during the IVF stimulation process. None of the leptin levels differed based on pregnancy outcome. BMI significantly correlated with all leptin levels. Follicular fluid estradiol correlated with serum estradiol only in pregnant patients (r = 0.97, p<0.01) and was unrelated in non-pregnant patients (r=−0.15, p=0.81).

Conclusion: Serum and follicular leptin levels are highly correlated. Leptin levels increase during the IVF cycle and vary between patients based on maternal BMI, but do not correlate with other serum hormone levels or pregnancy outcome. Pregnancy outcome success was reflected in the relationship between follicular fluid and serum levels of estradiol, independent of leptin levels.

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References

  1. Garcia-Mayor RV, Andrade MA, Rios M, Lage M, Dieguez C, Casanueva FF. Serum leptin levels in normal children: relationship to age, gender, body mass index, pituitary-gonadal hormones, and pubertal stage. J Clin Endocrinol Metab 1997;82:2849–55.

    Article  PubMed  CAS  Google Scholar 

  2. Henson MC, Castracane VD, O’Neil JS, Gimpel T, Swan KF, Green AE, Shi W. Serum leptin concentrations and expression of leptin transcripts in placental trophoblast with advancing baboon pregnancy. J Clin Endocrinol Metab 1999;84:2543–9.

    Article  PubMed  CAS  Google Scholar 

  3. Sivan E, Whittaker PG, Sinha D, Homko CJ, Lin M, Reece EA, Boden G. Leptin in human pregnancy: the relationship with gestational hormones. Am J Obstet Gynecol 1998;179:1128–32.

    Article  PubMed  CAS  Google Scholar 

  4. Kitawaki J, Koshiba H, Koshiba H, Tsukamoto K, Honjo H. Expression of leptin receptor in human endometrium and fluctuation during the menstrual cycle. J Clin Endocrinol Metab 2000;89:1946–50.

    Article  Google Scholar 

  5. Gonzalez RR, Simon C, Caballero-Campo P, Norman R, Chardonnens D, Devoto L, Bischof P. Leptin and reproduction. Human Reproduction Update 2000;6:290–300.

    Article  PubMed  CAS  Google Scholar 

  6. Imani B, Eijkemans MJ, de Jong FH, Payne NN, Bouchard P, Giudice LC, Fauser BC. Free androgen index and leptin are the most prominent endocrine predictors of ovarian response during clomiphene citrate induction of ovulation in normogonadotropic oligoamenorrheic infertility. J Clin Endocrinol Metab 2000;85:676–82.

    Article  PubMed  CAS  Google Scholar 

  7. Laughlin Ga, Morales AJ, Yen SS. Serum leptin levels in women with polycystic ovary syndrome: the role of insulin resistance/hyperinsulinemia. J Clin Endocrinol Metab 1997;82:1692–6.

    Article  PubMed  CAS  Google Scholar 

  8. Chen R, Fisch B, Ben-Haroush A, Kaplan B, Hod M, Orvieto R. Serum and follicular fluid leptin levels in patients undergoing controlled ovarian hyperstimulation for in vitro fertilization. Clin Exper Obstet Gynecol 2004;31:103–6.

    CAS  Google Scholar 

  9. Robins JC, Srivastava R, Mershon JL, Thomas MA. Association of leptin with poor ovarian hyperstimulation during in vitro fertilization. J Reprod Med 2005;50:356–60.

    PubMed  CAS  Google Scholar 

  10. Anifandis G, Koutselini E, Louridas K, Liakopoulos V, Leivaditis K, Mantzavinos T, Siotopoulou D, Vamvakopoulos N. Estradiol and leptin as conditional prognostic IVF markers. Reproduction 2005;129:531–4.

    Article  PubMed  CAS  Google Scholar 

  11. Asimakopoulos B, Nikolettos N, Papachristou DN, Simopoulou M, Al-Hasani S, Diedrich K. Follicular fluid levels of vascular endothelial growth factor and leptin are associated with pregnancy outcome or normal women participating in intracytoplasmic sperm injection cycles. Physiological Research 2005;54:263–70.

    PubMed  CAS  Google Scholar 

  12. Butzow T, Moilanen J, Lehtovitra M, Tuomi T, Hovatta O, Siegber R, Nilson CG, Apter D. Serum and follicular fluid leptin during in vitro fertilization: relationship among leptin increase, body fat mass, and reduced ovarian response. J Clin Endocrinol Metab 1999;84:3135–9.

    Article  PubMed  CAS  Google Scholar 

  13. Gurbuz B, Yalti S, Ficicioglu C, Tasdemir S. The relationship of serum and follicular fluid leptin and ovarian steroid levels in response to induction of ovulation in in vitro fertilization cycles. Eur J Obstet Gynecol Reprod Biol 2005;118:214–8.

    Article  PubMed  Google Scholar 

  14. Wunder DM, Kretschmer R, Bersinger NA. Concentrations of leptin and C-reactive protein in serum and follicular fluid during assisted reproductive cycles. Hum Reprod 2005;20:1266–71.

    Article  PubMed  CAS  Google Scholar 

  15. Welt CK, Heist K, Mantzoros CS. Leptin and soluble leptin receptor in follicular fluid. J Assist Reprod Genet 2003;20:495–501.

    Article  PubMed  Google Scholar 

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Acknowledgment

This study was supported by the Department of Clinical Investigation at Tripler Army Medical Center, Honolulu, HI.

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Authors and Affiliations

  1. Tripler Army Medical Center, Honolulu, Hawaii, USA

    Micah J. Hill, Catherine F. T. Uyehara, Glenn M. Hashiro & John L. Frattarelli

  2. Reproductive Medicine Associates of New Jersey, 100 Franklin Square Drive, Suite 200, Somerset, NJ, 08873, USA

    John L. Frattarelli

Authors
  1. Micah J. Hill
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  2. Catherine F. T. Uyehara
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  3. Glenn M. Hashiro
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  4. John L. Frattarelli
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Corresponding author

Correspondence to John L. Frattarelli.

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The views expressed in this manuscript are those of the authors and do not reflect the official policy or position of the Department of the Army, Department of Defense, or the U.S. Government.

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Hill, M.J., Uyehara, C.F.T., Hashiro, G.M. et al. The utility of serum leptin and follicular fluid leptin, estradiol, and progesterone levels during an in vitro fertilization cycle. J Assist Reprod Genet 24, 183–188 (2007). https://doi.org/10.1007/s10815-007-9106-0

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  • DOI: https://doi.org/10.1007/s10815-007-9106-0

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