Skip to main content
Log in

Birth after transfer of frozen-thawed vitrified biopsied blastocysts

  • Case Report
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose: To present a case describing the birth of a healthy female after the replacement of vitrified biopsied embryos after Preimplantation Genetic Diagnosis.

Method: A descriptive case report of a single patient.

Results: Our patient carrier of an X-linked disease became pregnant and as a result a healthy girl was born.

Conclusions: This report shows that blastocysts obtained from biopsied embryos can be successfully cryopreserved by a simple, secure and low-cost vitrification method using a Hemi-straw support.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Handyside AH, Kontogianni EH, Hardy K, Winston RM. Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature 1990;344:768–70

    Article  PubMed  CAS  Google Scholar 

  2. Joris H, Van Den AE, Vos AD, Van SA. Reduced survival after human embryo biopsy and subsequent cryopreservation. Hum Reprod 1999;14:2833–7

    Article  PubMed  CAS  Google Scholar 

  3. Magli MC, Gianaroli L, Fortini D, Ferraretti AP, Munne S. Impact of blastomere biopsy and cryopreservation techniques on human embryo viability. Hum Reprod 1999;14:770–3

    Article  PubMed  CAS  Google Scholar 

  4. Choi DH, Chung HM, Lim JM, Ko JJ, Yoon TK, Cha KY. Pregnancy and delivery of healthy infants developed from vitrified blastocysts in an IVF-ET program. Fertil Steril 2000;74:838–9

    Article  PubMed  CAS  Google Scholar 

  5. Mukaida T, Takahashi K, Kasai M. Blastocyst cryopreservation: ultrarapid vitrification using cryoloop technique. Reprod Biomed Online 2003;6:221–5

    Article  PubMed  Google Scholar 

  6. Reed ML, Lane M, Gardner DK, Jensen NL, Thompson J. Vitrification of human blastocysts using the cryoloop method: successful clinical application and birth of offspring. J Assist Reprod Genet 2002;19:304–6

    Article  PubMed  Google Scholar 

  7. Stehlik E, Stehlik J, Katayama KP, Kuwayama M, Jambor V, Brohammer R, Kato O. Vitrification demonstrates significant improvement versus slow freezing of human blastocysts. Reprod Biomed Online 2005;11:53–7

    PubMed  Google Scholar 

  8. Vanderzwalmen P, Bertin G, Debauche C, Standaert V, Bollen N, van RE, Vandervorst M, Schoysman R, Zech N. Vitrification of human blastocysts with the Hemi-Straw carrier: application of assisted hatching after thawing. Hum Reprod 2003;18:1504–11

    Article  PubMed  CAS  Google Scholar 

  9. Zech NH, Lejeune B, Zech H, Vanderzwalmen P. Vitrification of hatching and hatched human blastocysts: effect of an opening in the zona pellucida before vitrification. Reprod Biomed Online 2005;11:355–61

    PubMed  CAS  Google Scholar 

  10. Galn A, Escribá MJ, Gámiz P, Mercader A, Rubio C, Crespo J. High survival rate of human blastocysts after preimplantation genetic diagnosis and vitrification. Hum Reprod 2003;18(Suppl 1):xviii140–xviii157.

    Google Scholar 

  11. Boada M, Carrera M, De La Iglesia C, Sandalinas M, Barri PN, Veiga A. Successful use of a laser for human embryo biopsy in preimplantation genetic diagnosis: report of two cases. J Assist Reprod Genet 1998;15:302–7

    Article  PubMed  CAS  Google Scholar 

  12. Zheng WT, Zhuang GL, Zhou CQ, Fang C, Ou JP, Li T, Zhang MF, Liang XY. Comparison of the survival of human biopsied embryos after cryopreservation with four different methods using non-transferable embryos. Hum Reprod 2005;20:1615–8

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to M. Parriego.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Parriego, M., Solé, M., Aurell, R. et al. Birth after transfer of frozen-thawed vitrified biopsied blastocysts. J Assist Reprod Genet 24, 147–149 (2007). https://doi.org/10.1007/s10815-006-9094-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10815-006-9094-5

Keywords

Navigation