Effect of micro-environment maintenance on embryo culture after in-vitro fertilization: comparison of top-load mini incubator and conventional front-load incubator
- 286 Downloads
Purpose: To investigate the effect of microenvironment maintenance on embryo culture and clinical results using two types of incubators.
Methods: Temperature and oxygen concentration in a mini-incubator and a conventional incubator were compared following a 5-s door opening/closing procedure. Embryos of 30 in-vitro fertilization embryo transfer (IVF-ET) cases were randomly allocated to either one of the incubator, cultured, and the early-stage good embryo formation rate and the good blastocyst formation rate were compared, as indicators for micro-environment maintenance ability.
Results: Temperature recovery after a 5-s door opening/closing procedure was approximately 5 min for the mini-incubator and 30 min for the conventional incubator. The oxygen concentration return was significantly improved in the mini-incubator (3.0 ± 0 min) compared with the conventional incubator (7.8 ± 0.9 min). Both the early-stage good embryo formation rate and the good blastocyst formation rate were significantly higher in the mini-incubator (39.5% and 15.1%) than the conventional incubator (28.4% and 7.8%).
Conclusion: The microenvironment maintenance ability of incubators appears to significantly influence the formation of good embryos.
KeywordsIVF-ET Incubator Micro-environment maintenance Embryo formation rate
- 2.Zollner KP, Zollner U, Schneider M, Dietl J, Steck T. Comparison of two media for sequential culture after IVF and ICSI shows no differences in pregnancy rate: a randomized trial. Med Sci Monit 2004;10:1–7Google Scholar
- 3.Gardner DK, Lane M. Culture systems for the human embryo. In DK Gardner, A Weissman, CM Howles, Z Shoham (eds), Textbook of Assisted Reproductive Techniques, 2nd edn, London: Taylor & Francis, 2004, pp 211–34Google Scholar
- 5.Hadachi Y, Kato Y, Genji Y, Ueno Y, Nishikori K, Mio Y. Effect of optimal temperature in culture medium on embryo quality and ART result in couples with elder maternal age. J Fertil Implant (Tokyo) 2003;20:114–7Google Scholar
- 7.Gardner DK, Schoolcraft WB. In vitro culture of human blastcysts. In: R Jansen, D Mortimer (eds), Towards Reproductive Certainty: Fertility and Genetics Beyond 1999, Carnforth, UK: Parthenon Publishing, 1999, pp 378–88Google Scholar
- 9.Mortimer D, Henman M, Catt JW, Bowman MC, Jansen RPS. Development of an improved IVF culture system. SYDNEY IVF MEDIA ABSTRACTS, pp. 8–9, 2006Google Scholar