Light exposure of the ovum and preimplantation embryo during ART procedures

  • Lars D. M. Ottosen
  • Johnny Hindkjær
  • Jakob Ingerslev
Brief Communication


Exposure to visible light (400–700 nm wavelengths) is an unnatural stress factor to preimplantation embryos cultured in vitro. This study investigated the spectral composition and intensity of light during IVF procedures, and calculated radiation doses reaching the embryo during handling and manipulation. The study shows that normal IVF procedure may result in stressing radiation doses, unless filters are applied. This is at present not sufficiently recognised. No Danish IVF clinics use filters to protect embryos against visible light. 95% of the radiation was from microscopes. Ambient light, in contrast, was not a significant contributor to light stress and the use of dark laboratories is not justified.


Blue light filter Light energy exposure In vitro culture Preimplantation embryo Visible light 



This work was supported by the Danish Medical Research Council, The Beckett Foundation, The Foundation of 17/12-1981, The Toyota Foundation, the A.P. Moller Foundation for the advancement of Medical Science and Clinical Institute Aarhus University.


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Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Lars D. M. Ottosen
    • 1
  • Johnny Hindkjær
    • 1
  • Jakob Ingerslev
    • 1
  1. 1.The Fertility Clinic, Department of Gynecology and ObstetricsAarhus University Hospital Skejby SygehusÅrhusDenmark

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