Effect of rescuing donated immature human oocytes derived after FSH/hCG stimulation following in vitro culture with or without Follicular Fluid Meiosis Activating Sterol (FF-MAS)—an embryo chromosomal and morphological analysis
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Purpose: Studies in mice and humans have shown that Follicular Fluid – Meiosis Activating Sterol (FF-MAS) induces meiotic maturation of immature oocytes in vitro. A multicenter, prospectively randomised study evaluated chromosomal status of embryos from FSH/hCG primed human immature oocytes, cultured with or without FF-MAS.
Methods: Denuded immature oocytes (n=365) were randomly allocated into inert control, FF-MAS 5 μM or 20 μM. Seventy ±2 hours after ICSI on matured oocytes, all cleaved embryos were fixed for fluorescence in situ hybridisation analysis.
Results: Only 15% of oocytes resulted in cleaved embryos. GV oocytes matured at significantly lower rates (14% and 7%) in the two FF-MAS groups compared to the inert control group (47%). High rates of chromosomal abnormalities were found in all groups.
Conclusion: Immature oocytes showed poor development with high rates of embryo chromosomal abnormalities. Exposure to FF-MAS in the concentrations, duration and/or formulation used in this study did not improve the results.
KeywordsImmature oocytes FF-MAS Human Aneuploidy In vitro fertilisation
We would like to thank the staff at the IVF units at Sahlgrenska University Hospital, Rigshospitalet, Carlanderska Hospital as well as Quintiles AB and Quest Diagnostics for great support. At Novo Nordisk we received generous help from Lisbeth Helmgaard, Søren Larsen, Hansoo Kim and Lars Müller.
- 15.Ziebe S, Lundin K, Loft A, Bergh C, Nyboe Andersen A, Selleskog U, Nielsen D, Grondahl C, Kim H, Arce JC. CEMAS II and Study Group FISH analysis for chromosomes 13, 16, 18, 21, 22, X and Y in all blastomeres of IVF pre-embryos from 144 randomly selected donated human oocytes and impact on pre-embryo morphology. Hum Reprod 2003;18:2575–81PubMedCrossRefGoogle Scholar
- 18.Bergh C, Loft A, Lundin K, Ziebe S, Nilsson L, Wikland M, Grondahl C, Arce JC. CEMAS II Study Group: Chromosomal abnormality rate in human pre-embryos derived from in vitro fertilization cycles cultured in the presence of Follicular-Fluid Meiosis Activating Sterol (FF-MAS). Hum Reprod 2004;19:2109–17PubMedCrossRefGoogle Scholar
- 19.Loft A, Bergh C, Ziebe S, Lundin K, Andersen AN, Wikland M, Kim H, Arce JC. A randomized, double-blind, controlled trial of the effect of adding follicular fluid meiosis activating sterol in an ethanol formulation to donated human cumulus-enclosed oocytes before fertilization. Fertil Steril 2004;81:42–50PubMedCrossRefGoogle Scholar
- 20.Loft A, Ziebe S, Erb K, Rasmussen PE, Agerholm I, Hauge B, Bungum M, Bungum L, Grondahl C, Lyby K. Impact of follicular-fluid meiosis-activating sterol in an albumin-based formulation on the incidence of human pre-embryos with chromosome abnormalities. Fertil Steril 2005;84 Suppl 2:1269–76PubMedCrossRefGoogle Scholar