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Expression of inhibin-activin subunits, follistatin and smads in granulosa-luteal cells collected at oocyte retrieval

  • Shiuh Young Chang
  • Hong-Yo Kang
  • Kuo-Chung Lan
  • Chang-Yi Hseh
  • Fu-Jen Huang
  • Ko-En Huang
Original Paper

Abstract

Purpose: To examine the expression of inhibin-activin subunits, follistatin, and Smads 2, 3 and 4 in granulosa-luteal cells at the time of oocyte retrieval.

Methods: Quantitative reverse transcriptase-polymerase chain reaction was performed to quantify the mRNA expression of the investigated genes: inhibin α-subunit, inhibin-activin βA- and βB-subunits, follistatin, and Smads 2, 3 and 4.

Results: α-, βA- and βB-subunits of inhibin-activin, and follistatin were all well expressed in granulosa-luteal cells obtained from either dominant or cohort follicles. α-subunits strongly correlated with Smad2 (p < 0.001). βA-subunit significantly correlated with Smad4, p < 0.001. βB-subunit significantly correlated with Smad2, p=0.002, and follistatin expression strongly correlated with the expression of Smads 2 and 4 (p < 0.001 and = 0.007 respectively).

Conclusions: We found, for the first time that inhibin-activin βB-subunit mRNA was well expressed in human granulosa-luteal cells obtained from either dominant or cohort follicles. α-, βA- and βB-subunits and follistatin correlated with Smads to varied degrees, suggesting the active roles of the above genes at the time of oocyte retrieval.

Keywords

Activin Follistatin Granulosa cells Inhibin Smad 

Notes

Acknowledgments

This study was supported by grant NSC 92-2314-B-182-084 from the National Science Council, Taiwan.

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Copyright information

© Springer Science&#x002B;Business Media, LLC 2006

Authors and Affiliations

  • Shiuh Young Chang
    • 1
    • 2
  • Hong-Yo Kang
    • 2
    • 3
  • Kuo-Chung Lan
    • 1
    • 2
  • Chang-Yi Hseh
    • 3
  • Fu-Jen Huang
    • 1
    • 2
  • Ko-En Huang
    • 1
    • 2
    • 3
  1. 1.Department of Obstetrics and GynecologyChang Gung Memorial Hospital-Kaohsiung Medical CenterKaohsiung CountyTaiwan
  2. 2.Chang Gung University College of MedicineTaoyuanTaiwan
  3. 3.Center of Reproductive MedicineChang Gung Memorial HospitalKaohsiungTaiwan

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