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Journal of Assisted Reproduction and Genetics

, Volume 23, Issue 4, pp 191–198 | Cite as

Correlation of somatic cell steroid secretion and quality of generated oocytes after in-vitro stimulation of mouse follicles

  • Hung-Ching LiuEmail author
  • Zhiying He
  • Zev Rosenwaks
Animal Experimentation

Abstract

Purpose: To test the possibility of follicular somatic cell steroidogenesis as a marker for quality of their embraced oocytes.

Methods: Mechanically isolated mouse preantral follicles were cultured and matured in-vitro (IVC/IVM) for study.

Results: During IVC/IVM, oogenesis occurred concomitantly with folliculogenesis in a coordinated manner and simultaneously with progressive increments of somatic cell steroidogenesis. Follicular E2 production of matured oocytes were significantly higher than that of immature ones. The majority of MII oocytes (32/36) and all developed blastocysts(12/12) were associated with active E2 production prior to ovulation. In this study, 18 MII oocytes met both requirements for active and optimal E2 production. 13 of them were fertilized and 10 developed into blastocysts.

Conclusion: Active somatic cell steroidogenesis prior to ovulation and an optimal steroid milieu at ovulation are prerequisites for generation of competent oocytes after follicular maturation in-vitro.

Keywords

Follicular in-vitro maturation Oocyte competency Somatic cell steroidogenesis 

References

  1. 1.
    Liu HC, He ZY, Rosenwaks Z. In-vitro culture and in-vitro maturation of mouse preantral follicles with recombinant gonadotropins. 2002;77 (2):373–383Google Scholar
  2. 2.
    Liu HC, He ZM, Rosenwaks Z. Mouse ovarian cryopreservation has only a minor effect on in-vitro follicular maturation and gene expression. J Assist Reprod Genet 2003;20 (10):421–431CrossRefPubMedGoogle Scholar
  3. 3.
    Cortvridt R, Hu Y, Smitz J. Recombinant luteinizing hormone as a survival and differentiation factor increases oocyte maturation in recombinant follicle stimulating hormone-supplemented mouse preantral follicle culture. Hum Reprod 1998;13 (5):1292–1302CrossRefPubMedGoogle Scholar
  4. 4.
    Cortvrindt R, Smitz J, Van Steirteghem AC. Assessment of the need for follicle stimulating hormone in early preantral mouse follicle culture in-vitro. Hum Reprod 1997;12 (4):759–768CrossRefPubMedGoogle Scholar
  5. 5.
    Hasegawa A, Hamada Y, Mehandjiev T, Koyama K. In-vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries. Fertil Steril 2004;81 (1):824–830CrossRefPubMedGoogle Scholar
  6. 6.
    Cortvrindt RG, Hu YX, Liu J, Smitz J. Timed analysis of the nuclear maturation of oocytes in early preantral mouse follicle culture supplemented with recombinant gonadotropin. Fertil Steril 1998;70 (6):1114–1125CrossRefPubMedGoogle Scholar
  7. 7.
    Boland NI, Humpherson PG, Leese HJ, Gosden RG. The effect of glucose metabolism on murine follicle development and steroidogenesis in-vitro. Human Reprod 1994;(9):617–623Google Scholar
  8. 8.
    Eppig JJ, O’Brien MJ. Development in-vitro of mouse oocytes from primordial follicles. Biol Reprod 1996; (54):197–207CrossRefPubMedGoogle Scholar
  9. 9.
    De La Fuente R, O’Brien MJ, Eppig JJ. Epidermal growth factor enhances preimplantation developmental competence of maturing mouse oocytes. Hum Reprod 1999;14 (12):3060–3068CrossRefPubMedGoogle Scholar
  10. 10.
    Smitz J, Cortvrindt R. Oocyte in-vitro maturation and follicle culture: current clinical achievements and future directions. Hum Reprod 1999;14 (1):145–161PubMedGoogle Scholar
  11. 11.
    Wood TC, Montali RJ, Wildt DE. Follicle-oocyte atresia and temporal taphonomy in cold-stored domestic cat ovaries. Mol Reprod Dev 1997;46 (2):190–200CrossRefPubMedGoogle Scholar
  12. 12.
    Hendriksen PJ, Vos PL, Steenweg WN, Bevers MM, Dieleman SJ. Bovine follicular development and its effect on the in-vitro competence of oocytes. Theriogenol 2000;53 (1):11–20CrossRefGoogle Scholar
  13. 13.
    Hagemann LJ. Influence of the dominant follicle on oocytes from subordinate follicles. Theriogenology 1999;51 (2):449–459CrossRefPubMedGoogle Scholar
  14. 14.
    Funahashi H, Day BN. Advances in in-vitro production of pig embryos. J Reprod Fertil Suppl 1997; (52):271–283PubMedGoogle Scholar
  15. 15.
    Picton HM, Danfour MA, Harris SE, Chambers EL, Huntriss J. Growth and maturation of oocytes in-vitro. Reprod Suppl 2003; 61445–61462Google Scholar
  16. 16.
    Telfer EE, Binnie JP, McCaffery FH, Campbell BK. In-vitro development of oocytes from porcine and bovine primary follicles. Mol Cell Endocrinol 2000;163 Issue 1– (2):117–123CrossRefPubMedGoogle Scholar
  17. 17.
    Wright CS, Hovatta O, Margara R, Trew G, Winston RM, Franks S, et al. Effects of follicle-stimulating hormone and serum substitution on the in-vitro growth of human ovarian follicles. Hum Reprod 1999;14 (6):1555–1562CrossRefPubMedGoogle Scholar
  18. 18.
    Smitz J, Cortvrindt R. Follicle culture after ovarian cryostorage. Maturitas 1998;30 (2):171–179CrossRefPubMedGoogle Scholar
  19. 19.
    Senbon S, Hirao Y, Miyano T. Interactions between the oocyte and surrounding somatic cells in follicular development: lessons from in-vitro culture. J Reprod Dev 2003;49 (4):259–269CrossRefPubMedGoogle Scholar
  20. 20.
    Oktay K, Briggs D, Gosden RG. Ontogeny of follicle stimulating hormone receptor gene expression in isolated human ovarian follicles. J Clin Endocrinol Metab 1997;(82):3748–3751CrossRefPubMedGoogle Scholar
  21. 21.
    McNatty KP, Heath DA, Lundy T, Fidler AE, Quirke L, O’Connell A, et al. Control of early ovarian follicular development. J Reprod Fertil Suppl 1999;(49):123–135Google Scholar
  22. 22.
    Moor RM, Dai Y, Lee C, Fulka J. Oocyte maturation and embryonic failure. Hum Reprod Update 1998;(4):223–236CrossRefPubMedGoogle Scholar
  23. 23.
    Fulka J, Jr, First NL, Moor RM. Nuclear and cytoplasmic determinants involved in the regulation of mammalian oocyte maturation. Mol Hum Reprod 1998;(4):41–49CrossRefPubMedGoogle Scholar
  24. 24.
    Eppig JJ, Schultz RM, O’Brien M, Chesnel F. Relationship between the developmental programs controlling nuclear and cytoplasmic maturation of mouse oocytes. Dev Biol 1994; (164):1–9CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Institute for Reproductive MedicineThe Center for Reproductive Medicine and Infertility, Weill Medical College of Cornell UniversityNew YorkUSA

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