Purpose: To test the possibility of follicular somatic cell steroidogenesis as a marker for quality of their embraced oocytes.
Methods: Mechanically isolated mouse preantral follicles were cultured and matured in-vitro (IVC/IVM) for study.
Results: During IVC/IVM, oogenesis occurred concomitantly with folliculogenesis in a coordinated manner and simultaneously with progressive increments of somatic cell steroidogenesis. Follicular E2 production of matured oocytes were significantly higher than that of immature ones. The majority of MII oocytes (32/36) and all developed blastocysts(12/12) were associated with active E2 production prior to ovulation. In this study, 18 MII oocytes met both requirements for active and optimal E2 production. 13 of them were fertilized and 10 developed into blastocysts.
Conclusion: Active somatic cell steroidogenesis prior to ovulation and an optimal steroid milieu at ovulation are prerequisites for generation of competent oocytes after follicular maturation in-vitro.
This is a preview of subscription content, log in to check access.
Liu HC, He ZY, Rosenwaks Z. In-vitro culture and in-vitro maturation of mouse preantral follicles with recombinant gonadotropins. 2002;77 (2):373–383Google Scholar
Liu HC, He ZM, Rosenwaks Z. Mouse ovarian cryopreservation has only a minor effect on in-vitro follicular maturation and gene expression. J Assist Reprod Genet 2003;20 (10):421–431CrossRefPubMedGoogle Scholar
Cortvridt R, Hu Y, Smitz J. Recombinant luteinizing hormone as a survival and differentiation factor increases oocyte maturation in recombinant follicle stimulating hormone-supplemented mouse preantral follicle culture. Hum Reprod 1998;13 (5):1292–1302CrossRefPubMedGoogle Scholar
Cortvrindt R, Smitz J, Van Steirteghem AC. Assessment of the need for follicle stimulating hormone in early preantral mouse follicle culture in-vitro. Hum Reprod 1997;12 (4):759–768CrossRefPubMedGoogle Scholar
Hasegawa A, Hamada Y, Mehandjiev T, Koyama K. In-vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries. Fertil Steril 2004;81 (1):824–830CrossRefPubMedGoogle Scholar
Cortvrindt RG, Hu YX, Liu J, Smitz J. Timed analysis of the nuclear maturation of oocytes in early preantral mouse follicle culture supplemented with recombinant gonadotropin. Fertil Steril 1998;70 (6):1114–1125CrossRefPubMedGoogle Scholar
Boland NI, Humpherson PG, Leese HJ, Gosden RG. The effect of glucose metabolism on murine follicle development and steroidogenesis in-vitro. Human Reprod 1994;(9):617–623Google Scholar
Eppig JJ, O’Brien MJ. Development in-vitro of mouse oocytes from primordial follicles. Biol Reprod 1996; (54):197–207CrossRefPubMedGoogle Scholar
De La Fuente R, O’Brien MJ, Eppig JJ. Epidermal growth factor enhances preimplantation developmental competence of maturing mouse oocytes. Hum Reprod 1999;14 (12):3060–3068CrossRefPubMedGoogle Scholar
Smitz J, Cortvrindt R. Oocyte in-vitro maturation and follicle culture: current clinical achievements and future directions. Hum Reprod 1999;14 (1):145–161PubMedGoogle Scholar
Wood TC, Montali RJ, Wildt DE. Follicle-oocyte atresia and temporal taphonomy in cold-stored domestic cat ovaries. Mol Reprod Dev 1997;46 (2):190–200CrossRefPubMedGoogle Scholar
Hendriksen PJ, Vos PL, Steenweg WN, Bevers MM, Dieleman SJ. Bovine follicular development and its effect on the in-vitro competence of oocytes. Theriogenol 2000;53 (1):11–20CrossRefGoogle Scholar
Hagemann LJ. Influence of the dominant follicle on oocytes from subordinate follicles. Theriogenology 1999;51 (2):449–459CrossRefPubMedGoogle Scholar
Funahashi H, Day BN. Advances in in-vitro production of pig embryos. J Reprod Fertil Suppl 1997; (52):271–283PubMedGoogle Scholar
Picton HM, Danfour MA, Harris SE, Chambers EL, Huntriss J. Growth and maturation of oocytes in-vitro. Reprod Suppl 2003; 61445–61462Google Scholar
Telfer EE, Binnie JP, McCaffery FH, Campbell BK. In-vitro development of oocytes from porcine and bovine primary follicles. Mol Cell Endocrinol 2000;163 Issue 1– (2):117–123CrossRefPubMedGoogle Scholar
Wright CS, Hovatta O, Margara R, Trew G, Winston RM, Franks S, et al. Effects of follicle-stimulating hormone and serum substitution on the in-vitro growth of human ovarian follicles. Hum Reprod 1999;14 (6):1555–1562CrossRefPubMedGoogle Scholar
Senbon S, Hirao Y, Miyano T. Interactions between the oocyte and surrounding somatic cells in follicular development: lessons from in-vitro culture. J Reprod Dev 2003;49 (4):259–269CrossRefPubMedGoogle Scholar
Oktay K, Briggs D, Gosden RG. Ontogeny of follicle stimulating hormone receptor gene expression in isolated human ovarian follicles. J Clin Endocrinol Metab 1997;(82):3748–3751CrossRefPubMedGoogle Scholar
McNatty KP, Heath DA, Lundy T, Fidler AE, Quirke L, O’Connell A, et al. Control of early ovarian follicular development. J Reprod Fertil Suppl 1999;(49):123–135Google Scholar
Fulka J, Jr, First NL, Moor RM. Nuclear and cytoplasmic determinants involved in the regulation of mammalian oocyte maturation. Mol Hum Reprod 1998;(4):41–49CrossRefPubMedGoogle Scholar
Eppig JJ, Schultz RM, O’Brien M, Chesnel F. Relationship between the developmental programs controlling nuclear and cytoplasmic maturation of mouse oocytes. Dev Biol 1994; (164):1–9CrossRefPubMedGoogle Scholar