Journal of Assisted Reproduction and Genetics

, Volume 23, Issue 4, pp 185–189 | Cite as

Histologic characteristics of the endometrium predicts success when utilizing autologous endometrial coculture in patients with IVF failure

  • S. D. SpandorferEmail author
  • R. Soslow
  • R. Clark
  • S. Fasouliotis
  • O. K. Davis
  • Z. Rosenwaks
Assisted Reproduction

Purpose: To analyze the success of autologous endometrial coculture (AECC) in improving embryo quality and pregnancy outcome based on the histologic characteristic of the biopsy.

Methods: Prospective study of 86 consecutive patients undergoing IVF utilizing AECC.

Results: The patients were on average 37.4±4.0 years with a history of 2.6±1.8 failed previous attempts. An overall clinical pregnancy rate of 45.3% per ET was found. The embryos grown in AECC were of an improved quality in comparison to those grown in conventional media. 33.7% (29/86) of the biopsies were out of phase (>3 days). In-phase (IP) and OOP (out of phase) specimens both demonstrated an improvement in embryo quality. However, OOP endometrial biopsies that displayed significant retarded endometrial development (< cycle day 19) did not demonstrate an improvement in embryos grown on AECC as compared to IP endometrial biopsies or OOP endometrial biopsies that demonstrated at least an endometrial development of cycle day 19.

Conclusions: We have demonstrated a significant improvement in embryo quality with AECC. We have also demonstrated that histologic dating of the endometrium is predictive of IVF outcome when utilizing AECC.


Autologous endometrial coculture cytokines endometrium IVF. 


  1. 1.
    Barmat LI, Liu H-C, Spandorfer SD, Damario M, Veeck C, Rosenwaks Z: Human preembryo development on autologous endometrial coculture versus conventional medium: A randomized trial. Fertil and Steril 1998;70:1109–1113CrossRefGoogle Scholar
  2. 2.
    Barmat LI, Liu H-C, Spandorfer SD, Mele C, Veeck C, Rosenwaks Z: Autologous endometrial coculture in patients with repeated failure of implantation after IVF-ET. J Assist Reprod Genet 1999;16(3):121–127.PubMedCrossRefGoogle Scholar
  3. 3.
    Spandorfer SD, Barmat LI, Navarro J, Liu H-C, Veeck L, Rosenwaks Z: Autologous endometrial coculture in the patient with multiple implantation failures: The importance of the date of the biopsy. Fertil Steril 2002;77:1209–1213.Google Scholar
  4. 4.
    Spandorfer SD, Barmat LI, Liu H-C, Mele C, Veeck L, Rosenwaks Z: Granulocyte Macrophage-Colony Stimulating Factor production by autologous endometrial co-culture is associated with outcome for IVF patients with a history of multiple implantation failures. Am J Reprod Immunol 1998;40:377–381.PubMedGoogle Scholar
  5. 5.
    Spandorfer SD, Clarke R, Bovis L, Liu H-C, Neuer A, Witkin SS, Rosenwaks Z: Interleukin-1 levels in the supernatant of conditioned media of embryos grown in autologous endometrial coculture: Correlation with embryonic development and outcome for patients with a history of multiple implantation failures after IVF. Am J Reprod Immunol 2000;43(1):6–11.PubMedCrossRefGoogle Scholar
  6. 6.
    6. Spandorfer SD, Navarro J, Levy D, Black AR, Liu HC, Veeck L, Witkin SS, Rosenwaks Z: Autologous endometrial coculture in patients with in vitro-fertilization (IVF) failure: Correlations of outcome with leukemia inhibiting factor production. Am J Reprod Immunol 46:375–380Google Scholar
  7. 7.
    Noyes RW, Hertig G, Rock J: Dating the endometrial biopsy. Am J Obstet Gynecol 1975;122(2):262–3.PubMedGoogle Scholar
  8. 8.
    Davis OK, Rosenwaks Z: In vitro fertilization. In: Reproductive Endocrinology, Surgery, and Technology; Eli Adashi, John A Rock, Z Rosenwaks (ed), Philadelphia, Lippincott-Raven, 1996, pp 2319–2334.Google Scholar
  9. 9.
    Hill JA. Maternal-embryonic cross-talk. Ann NY Acad Sci 2001;943:17–25.PubMedCrossRefGoogle Scholar
  10. 10.
    Dimitriadis E, White CA, Jones RL, Salamonsen LA. Cytokines, chemokines and growth factors in endometrium related to implantation Hum Reprod Update 2005;11:613–630Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • S. D. Spandorfer
    • 1
    • 3
    Email author
  • R. Soslow
    • 2
  • R. Clark
    • 1
  • S. Fasouliotis
    • 1
  • O. K. Davis
    • 1
  • Z. Rosenwaks
    • 1
  1. 1.Department of Obstetrics and GynecologyCenter for Reproductive Medicine and Infertility, Cornell University Medical CenterNew York,USA
  2. 2.Department of PathologyCenter for Reproductive Medicine and Infertility, Cornell University Medical CenterNew York,USA
  3. 3.The Center for Reproductive Medicine and InfertilityThe New York Hospital/Cornell Medical CenterNew YorkUSA

Personalised recommendations