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Human papilloma virus DNA exposure and embryo survival is stage-specific

  • Assisted Reproduction
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Purpose: Human papillomavirus (HPV) has been shown to disrupt late-stage implanting embryos. The objectives were (a) to assess the development of early embryos exposed to HPV DNA and (b) to analyze the blastocyst hatching process after HPV exposure.

Methods: The study involved exposing two-cell and 4–8-cell mouse embryos to DNA fragments from either HPV type 16, type 18 or DQA1 (control). The embryos were incubated for 120 h and assessed.

Results: HPV 16 and 18 inhibited two-cell embryo development. In contrast, delaying the exposure of HPV DNA until the 4–8-cell stage resulted in further embryonic development. There was 25.9% less blastocyst formed with HPV 16 exposure. Additionally, there were 25.9–31.8% more degenerated embryos with HPV 16 exposure.

Conclusions: The study demonstrated embryo stage-specific effects of HPV on early development. The results suggested HPV exposure was linked to two-cell embryo demise and delaying the exposure of HPV until later embryo stages permitted embryo development. HPV 16 was shown to decrease blastocyst formation while HPV 18 inhibited the blastocyst hatching process.

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Authors and Affiliations

  1. Department of Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, California, USA

    Andrew A. Henneberg, William C. Patton, John D. Jacobson & Philip J. Chan

  2. Departments of Physiology and Pharmacology, Loma Linda University School of Medicine, Loma Linda, California, USA

    Philip J. Chan

  3. Loma Linda University Center for Fertility and In Vitro Fertilization, 11370 Anderson Street, Suite 3950, Loma Linda, 92354, California, USA

    Philip J. Chan

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  1. Andrew A. Henneberg
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  2. William C. Patton
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  3. John D. Jacobson
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  4. Philip J. Chan
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Correspondence to Philip J. Chan.

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Henneberg, A.A., Patton, W.C., Jacobson, J.D. et al. Human papilloma virus DNA exposure and embryo survival is stage-specific. J Assist Reprod Genet 23, 255–259 (2006). https://doi.org/10.1007/s10815-006-9030-8

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  • DOI: https://doi.org/10.1007/s10815-006-9030-8

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