Background : Intracytoplasmic sperm injection (ICSI) has risen to the forefront of reproductive technology. In the present study, the location of the sperm injection was noted, and a prospective study was conducted to evaluate the effect of the sperm retention site on cleavage rates and embryo quality after ICSI.
Methods : This study involved 336 ICSI patients (age 27–44; average 37.4) where 1545 oocytes were observed. An oocyte was divided into nine sites and the sperm retention site was observed microscopically after injection. The polar body was placed at either the twelve or six o’clock position. The injection pipette was introduced at the three o’clock position and oolemma rupture was ascertained by mild suction. The main outcome measures were the relationship of sperm remaining in position in the oocyte to fertilization rate and embryo quality.
Results : When the injection pipette was introduced at the three o’clock position, about 80% of the sperm remained in the center or left of center. The fertilization rate was significantly lower (p < 0.05) when the sperm remained near the site of introduction. Embryo quality was not significantly affected by the sperm retention site.
Conclusions : About 12–14% of the spermatozoa remained near the introducing position, and in these cases the fertilization rate was low. However, once fertilization occurred, the sperm retention site had minimal impact on embryo quality. Injecting sperm near the spindle site may improve embryo quality.
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REFERENCES
Palermo G, Joris H, Devroey P, Van Steirteghem AC: Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992;340:17–18
Cohen J, Scott R, Alikani M, et al.: Ooplasmic transfer in mature human oocytes. Mol Hum Reprod 1998;4:269–280
Van Steirteghem AC, Nagy Z, Joris H, et al.: High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod 1993;8:1061–1066
Nagy ZP, Joris H, Liu J, Staessen C, Devroey P, Van Steirteghem AC: Intracytoplasmic single sperm injection of 1-day-old unfertilized human oocytes. Hum Reprod 1993;8:2180–2184
Blake M, Garrisi J, Tomkin G, Cohen J: Sperm deposition site during ICSI affects fertilization and development. Fertil Steril 2000;73:31–37
Rienzi L, Ubaldi F, Martinez F, et al.: Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI. Hum Reprod 2003;18:1289–1293
Cooke S, Tyler JP, Driscoll GL: Meiotic spindle location and identification and its effect on embryonic cleavage plane and early development. Hum Reprod 2003;18:2397–2405
Avery S, Blayney M: Effect of the position of the meiotic spindle on the outcome of intracytoplasmic sperm injection. Hum Fertil (Camb) 2003;6:19–22
Dumoulin JM, Coonen E, Bras M, et al.: Embryo development and chromosomal anomalies after ICSI: Effect of the injection procedure. Hum Reprod 2001;16:306–312
Van Der Westerlaken LA, Helmerhorst FM, Hermans J, Naaktgeboren N: Intracytoplasmic sperm injection: Position of the polar body affects pregnancy rate. Hum Reprod 1999;14:2565–2569
Nagy ZP, Liu J, Joris H, et al.: The influence of the site of sperm deposition and mode of oolemma breakage at intracytoplasmic sperm injection on fertilization and embryo development rates. Hum Reprod 1995;10:3171–3177
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Yanaihara, A., Iwasaki, S., Negishi, M. et al. Sperm retention site and its influence on cleavage rate and early development following intracytoplasmic sperm injection. J Assist Reprod Genet 23, 63–67 (2006). https://doi.org/10.1007/s10815-006-9028-2
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DOI: https://doi.org/10.1007/s10815-006-9028-2