External quality control program for semen analysis: Spanish experience
- 80 Downloads
Purpose: Results from an external quality control programme for semen analysis carried out in Spain are analysed.
Methods: Quality control materials were distributed and the following seminal parameters were determined: concentration, total motility, progressive motility, rapid progressive motility, morphology and sperm vitality. The between-laboratories coefficients of variation were assessed on different types of quality control material.
Results: The majority of participating laboratories utilised manual versus computer-assisted semen analysis methods. Some between-laboratories coefficients of variation ranges were: 20.8–33.8% for concentration (semen pool suspension); 13.9–19.2% for total motility (videotapes); 54.2–70.2% for sperm morphology (strict criteria using stained smears); and 9.8–41.1% for sperm vitality (stained smears). There was an inverse relation between mean percentage of sperm and coefficients of variation between laboratories for sperm motility, morphology and vitality.
Conclusions: These data highlight the urgent need for improvement in the overall quality of andrology testing.
KeywordsExternal quality control semen analysis sperm concentration sperm morphology sperm motility
- 3.Clavert A, Bourguignat A, Férard G: Quality control in spermiology: The French experience. Symposium Abstracts, IFCC-Worldlab'99, Firenze, 6–11 June. Clin Chem Lab Med 1999;37:S1–S101Google Scholar
- 7.Auger J, Eustache F, Ducot B, Blandin T, Daudin M, Diaz I, El Matribi S, Gony B, Keskes L, Kolbezen M et al.: Intra- and inter-individuality variability in human sperm concentration, motility and vitality assessment during a workshop involving ten laboratories. Hum Reprod 2000;15:2360–2368Google Scholar
- 9.WHO: WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction, 4th ed. Cambridge, UK, Cambridge University Press, 1999Google Scholar
- 10.WHO: WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction, 3rd ed. Cambridge, UK, Cambridge University Press, 1992Google Scholar
- 12.Walker RH: Pilot surveys for proficiency testing of semen analysis. Arch Pathol Lab Med 1992;116:422–424Google Scholar
- 13.Carrell DT, Cornwell CE, Thorp C, Cartmill DC, Urry RL: Evaluation of a five year intra and interlaboratory semen analysis quality control program. Division of Urology, University of Utah School of Medicine, Salt Lake, 1987Google Scholar
- 14.Coetzee K, Kruger TF, Lombaard CJ, Shaughnessy D, Oehninger S, Ozgur K, Pomeroy KO, Muller CH, Muller C: Assessment of interlaboratory and intralaboratory sperm morphology readings with the use of a Hamilton Thorne Research integrated visual optical system semen analyzer. Fertil Steril 1999;71:80–84PubMedGoogle Scholar
- 15.Baker DJ, Paterson MA, Klaassen JM: Semen evaluations in the clinical laboratory. How well are they being performed? Lab Med 1994;25:509–514Google Scholar
- 17.Johnson JE, Blackhurst DW, Boone WR: Can Westgard quality control rules determine the suitability of frozen sperm pellets as a control material for computer assisted semen analyzers? J Assist Reprod Genet 2003;20:38–45Google Scholar
- 18.Kvist U, Björndahl L: ESHRE monographs: manual on basic semen analysis. Oxford, UK, Oxford University Press, 2002Google Scholar
- 20.Ombelet W, Bosmans E, Janssen M, Cox A, Maes M, Punjabi U, Blaton V, Gunst J, Haidl G, Wouters E et al.: Multicenter study on reproducibility of sperm morphology assessment. Arch Androl 1998;4:103–114Google Scholar