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Comparison of growth rates of fresh and frozen-thawed embryos according to chromosomal status

  • Rohini Edirisinghe
  • Rodney Jemmott
  • John Allan
Genetics

Abstract

Purpose: To report a comparison of growth rates between fresh and frozen-thawed embryos on day 3 and 4 according to chromosomal status during PGD.

Methods: Embryos were biopsied on day 3, fluorescent in situ hybridization performed for aneuploidy detection and growth rates monitored.

Results: Based on 241 egg pick up-PGD and 133 frozen embryo transfer-PGD cycles. The development rate of embryos despite their chromosomal status was similar on day 3, however, overall, fresh embryos showed a significantly improved development (≥6 cell stage; 85.7%) compared to frozen embryos (77.0%; p < 0.003). On day 4 fresh embryos showed accelerated development and further developmental improvements were associated with chromosomal normality (56.1% normal vs. 42.2% aneuploid; p < 0.004).

Conclusions: Cryopreservation slows embryo development regardless of the chromosomal status. However, chromosomal status did not influence the chance of embryo implantation which was the same for fresh and thawed embryos.

Key Words

Chromosomal status fresh embryos frozen embryos growth rates 

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Rohini Edirisinghe
    • 1
  • Rodney Jemmott
    • 1
  • John Allan
    • 1
  1. 1.The Wesley IVF ServiceThe Wesley HospitalQueenslandAustralia

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