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Clinical experiences of ICSI-ET thawing cycles with embryos cryopreserved at different developmental stages

  • Assisted Reproduction
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Abstract

Purpose: The aim of our study was to analyze factors including survival, implantation and pregnancy rate, patients’ age and BMI, abortions and extra uterine pregnancies that might influence the outcome of ICSI-ET thawing cycles.

Methods: A total of 147 cycles with embryos cryopreserved at different developmental stages were retrospectively evaluated.

Results: No difference was found in the survival, implantation and pregnancy rates of embryos cryopreserved on Day 2–3 and 5. However, in the pregnant group significantly higher implantation rate was observed with Day 5 blastocysts then with Day 2 or 3 early embryos. We found no difference in the number of abortions and extra uterine pregnancies between fresh and frozen ICSI-ET cycles. Higher BMI was found in the pregnant than in the non-pregnant group. However, the age of patient had no effect on the results.

Conclusions: Developmental stage of embryo and patients’ BMI influences the success of ICSI-ET thawing cycles.

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References

  1. Camus M: Human embryo cryopreservation: Review of clinical issues related to the success rate. Proceedings of Symposium on Cryobiology and Cryopreservation on Human Gametes and Embryos ESHRE Campus 2004, Brussels, Belgium–12 to 13 March 2004, pp. 24–26

  2. Hartshorne GM, Elder K, Crow J, Dyson H, Edwards RG: The influence of in vitro development upon post-thaw survival and implantation of cryopreserved blastocysts. Hum Reprod 1991;136–141

  3. Cohen J, Simons RF, Edwards RG, Fehilly CB, Fishel SB: Pregnancies following the frozen storage of expanding human blastocysts. J Vitro Fert Embryo Transfer 1985;2:59–64

    Article  CAS  Google Scholar 

  4. Testart J, Lassalle B, Belaisch-Allart J, Hazout A, Forman R, Rainhorn JD, Frydman R: High pregnancy rate after early human embryo freezing. Fertil Steril 1986;46:268–272

    CAS  PubMed  Google Scholar 

  5. Zeilmaker GH, Alberda A Th, van Gent I: Two pregnancies following transfer of intact frozen-thawed embryos. Fertil Steril 1984;42: 293–296

    CAS  PubMed  Google Scholar 

  6. Cohen J, De Vane GV, Elsner CW, Fehilly CB, Kort HI, Massey JB, Turner TG: Cryopreservation of zygotes and early cleaved human embryos. Fertil Steril 1988;49:283–289

    CAS  PubMed  Google Scholar 

  7. Mandelbaum J, Belaisch-Allart J, Junca AM, Antoine JM, Plachot M, Alvarez S, Alnot MO, Salat-Baroux J: Cryopreservation in human assisted reproduction is now routine for embryos but remains a research procedure for oocytes. Human Reprod 1988;13 (Suppl.):161–177

    Google Scholar 

  8. Edgar DH, Bourne H, Jericho H, Mcbain JC: The developmental poteintal of cryopreserved human embryos. Mol Cell Endocr 2000;169:69–72

    Article  CAS  Google Scholar 

  9. Kattera S, Shrivastau P, Craft I: Comparison of pregnancy outcome of pronuclear and multicellular stage frozen-thawed embryo transfers. J Assist Reprod Genet 1999;16:358–362

    Article  CAS  PubMed  Google Scholar 

  10. Trounson A, Mohr L: Human pregnancy following cryopreservation, thawing and transfer o fan eight-cell embryo. Nature 1983;305:707–709

    Article  CAS  PubMed  Google Scholar 

  11. Ménézo Y, Nicolet B, Herbaut N, Andre D: Freezing cocultured human blastocysts. Fertile Steril 1992;58:977–980

    Google Scholar 

  12. Anderson AR, Weikert ML, Crain JL: Determining the most optimal stage for embryo cryopreservation. Reprod Biomed Online 2004;8:207–211

    PubMed  Google Scholar 

  13. Gardner DK, Lane M, Stevens J, Schoolcraft WB: Changing the start temperature and cooling rate in a slow-freezing protocol increases human blastocyst viability. Fertil Steril 2003;79: 407–410

    Article  PubMed  Google Scholar 

  14. Veeck L: Does the developmental stage at freeze impact clinical results post-thaw? Reproductive BioMedicine Online 2003;6:367–374

    PubMed  Google Scholar 

  15. Kaufmann RA, Menezo Y, Hazout A, Nicollet B, Dumont M, Servy EJ: Cocultured blastocyst cryopreservation: experience of more than 500 transfer cycles. Fertil Steril 1995;64:358–362

    Google Scholar 

  16. Kolibianakis EM, Devroey P: Blastocyst culture: facts and fction. Reprod Biomed Online 2002;5:285–293

    PubMed  Google Scholar 

  17. Ménézo Y, Viega A: Cryopreservation of blastocysts. Proceedings of the 10th World Congress on IVF and Assisted reproduction. Vancouver, Canada, May 1997, pp. 41–45.

  18. Mandelbaum J, Ménézo Y: Embryo cryopreservation in humans. In A Comprehensive Textbook of Assisted Reproductive Technology: Laboratory and Clinical Perspective. DK Gardner, A. Weissman, CM Howles (eds), New York, Academic Press, 2001

    Google Scholar 

  19. Mandelbaum J: Human embryo cryopreservation: Past, present and future. Proceedings of Symposium on Cryobiology and Cryopreservation on Human Gametes and Embryos, ESHRE Campus 2004, Brussels, Belgium, 12 to 13 March 2004, pp. 17–22

  20. Miller JE, Smith TT: The effect on intracytoplasmic sperm injection and semen parameters on blastocyst development in vitro. Human Reprod 2001;16:918–924

    Article  CAS  Google Scholar 

  21. Blefco DA, Junca AM, Plachot M, Royere: Comparison between IVF and ICSI blastocyst culture and freezing: A French retrospective multicentre study. ESHRE, 2001, p. 170

  22. Kanyo Katalin, Konc J, Solti L, Cseh S: Assisted reproductive research: Laser assisted hatching and spindle detection (spindle view technique), Acta Veterinaria Hungarica 2004;52(1): 113–123

    Article  PubMed  Google Scholar 

  23. StatSoft Inc: (2004). STATISTICA (data analysis software system), version 6. http://www.statsoft.com

  24. Simon A, Holzer H, Hurwitz A, Revel A, Zentner BS, Lossos F, Laufer N: Comparison of cryopreservation outcome following intracytoplasmic sperm injection and conventional in vitro fertilization. J Assist Reprod Genet 1998;15:431–437

    Article  CAS  PubMed  Google Scholar 

  25. Kowalik A, Palermo GD, Barmat L, Veeck L, Rimarachin J, Rosenwaks Z: Comparison of clinical outcome after cryopreservation of embryos obtained from intracytoplasmic sperm injection and in vitro fertilization. Human Reprod 1998;13:2848–2851

    CAS  Google Scholar 

  26. Lahav-Baratz S, Koifman M, Shiloh H, Ishai D, Weiner-Megnazi Z, Dirnfeld M: Analyzing factors affecting the success rate of frozen-thawed embryos. J Assist Reprod Genet 2003;20:444–448.

    Article  CAS  PubMed  Google Scholar 

  27. Fugger EF: Clinical status of human embryo culture in the USA. Fertil Steril 1989;52:986–990

    CAS  PubMed  Google Scholar 

  28. Fugger EF, Bustillo M, Dorfmann AD, Schulman JP: Human preimplantation embryo culture: Selected aspects. Hum Reprod 1991;6:131–135.

    CAS  PubMed  Google Scholar 

  29. Catt JW, Ryan JP, Pike IL, O’Neill C, Saunders DM: Clinical intracytoplasmic sperm injection (ICSI) results from Royal North Shore. Reprod Fertil Dev 1995;7:255–262

    CAS  PubMed  Google Scholar 

  30. Payne D, Matthews CD: Intracytoplasmic sperm injection –Clinical results from the reproductive medicine unit, Adelaide. Reprod Fertil Dev 1995;7:219–227

    CAS  PubMed  Google Scholar 

  31. Al-Hasani S, Ludwig M, Gagstreiger F, Küpker W, Sturm R, Yilmaz A: Comparison of cryopreservation of supernumerary pronuclear human oocytes obtained after intracytoplasmic sperm injection (ICSI) and after conventional in vitro fertilization. Hum Reprod 1996;11:604–607

    CAS  PubMed  Google Scholar 

  32. Edwards RG, Brody SA: Principles and practice of assisted human reproduction. Philadelphia. W. B. Saunders Company, 1995

    Google Scholar 

  33. Wang JX, Davies M, Norman RJ: Body mass and probability of pregnancy during assisted reproduction treatment: Retrospective study. BMJ 2000; 321:1320–1321

    CAS  PubMed  Google Scholar 

  34. Winter E, Wang J, Davies MJ, Norman R: Early pregnancy loss following assisted reproductive technology treatment. Hum Reprod 2002;17:3220–3223

    Article  PubMed  Google Scholar 

  35. Ehrenberg HM, Dierker L, Milluzzi C, Mercer BM: Low maternal weight, failure to thrive in pregnancy, and advers pregnancy outcomes. Am J Obstet Gynecol 2003;189:1726–1730

    PubMed  Google Scholar 

  36. Wattanakumtornkul S, Damario MA, Stevens Hall SA, Thornhill AR, Tummon IS: Body mass index and uterine receptivity in the oocyte donation model. Fertil Steril 2003;80:336–340

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Infertility and IVF Center of Buda, Saint János Hospital, Budapest, 1125, Hungary

    Janos Konc, Katalin Kanyó & Sandor Cseh

  2. Laboratory for Andrology and Assisted Reproduction FVS, István u. 2, Budapest, Hungary, 1078

    Sandor Cseh

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  1. Janos Konc
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  2. Katalin Kanyó
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  3. Sandor Cseh
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Correspondence to Sandor Cseh.

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Konc, J., Kanyó, K. & Cseh, S. Clinical experiences of ICSI-ET thawing cycles with embryos cryopreserved at different developmental stages. J Assist Reprod Genet 22, 185–190 (2005). https://doi.org/10.1007/s10815-005-4920-8

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  • DOI: https://doi.org/10.1007/s10815-005-4920-8

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