Skip to main content
SpringerLink
Log in

Relationship between cytokines and the embryotoxicity of hydrosalpingeal fluid

  • Assisted Reproduction
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Objective: The exact chemical composition of hydrosalpingeal fluid is unknown. The objective of this study was to characterize cytokines in hydrosalpingeal fluid (HSF) and examine their possible role in the embryo development.

Study Design: HSF was aspirated at laparoscopic salpingectomy in eight infertile women. Levels of IL-1β, IL-13, IL-8, IL-6 and TNF-α in the HSF were determined by quantitative immunoassay kits. Two-cell mouse embryos were incubated with 0, 25, 50 and 75% concentrations of HSF. The blastocyst development rate (BDR) of mouse embryos was measured at each HSF concentration.

Result(s): The embryotoxicity of HSF was concentration dependent. An increase in the HSF concentration resulted in significant decrease in % BDR (p < 0.01). IL-1β was present in six of the eight HSF samples with a mean (± SD) concentration of 0.9 ± 0.8 pg/mL. IL-13 was not detected in any of the HSF samples. IL-8, IL-6 and TNF-α were detected in all samples with a mean (± SD) concentration of 4741.2 ± 6554.4 pg/mL, 204.8± 132.8 pg/ml and 12 ± 12.8 pg/mL respectively. IL-6 was positively correlated with BDR (r = 0.53; p < 0.04).

Conclusion(s): We demonstrated for the first time the presence of IL-1β, IL-8, IL-6 and TNF-α and the absence of IL-13 in human hydrosalpingeal fluid. IL-6 was positively related to the BDR.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Strandell A, Lindhard A, Waldenstrom U, Thorburn J, Janson PO, Hamberger L: Hydrosalpinx and IVF outcome: A prospective, randomized multicentre trial in Scandinavia on salpingectomy prior to IVF. Hum Reprod 1999;14:2762–2769

    CAS  PubMed  Google Scholar 

  2. Vandromme J, Chasse E, Lejeune B, Van Rysselberge M, Delvigne A, Leroy F: Hydrosalpinges in in-vitro fertilization: An unfavourable prognostic feature. Hum Reprod 1995;10:576–579

    CAS  PubMed  Google Scholar 

  3. Murray DL, Sagoskin AW, Widra EA, Levy MJ: The adverse effect of hydrosalpinges on in vitro fertilization pregnancy rates and the benefit of surgical correction. Fertil Steril 1998;69:41–45

    CAS  PubMed  Google Scholar 

  4. Nackley AC, Muasher SJ: The significance of hydrosalpinx in in vitro fertilization. Fertil Steril 1998;69:373–384

    CAS  PubMed  Google Scholar 

  5. Bredkjaer HE, Ziebe S, Hamid B, et al.: Delivery rates after in-vitro fertilization following bilateral salpingectomy due to hydrosalpinges: A case control study. Hum Reprod 1999;14:101–105

    CAS  PubMed  Google Scholar 

  6. Sharara FI: Effects of hydrosalpinx on IVF outcome: The search goes on. Hum Reprod 1997;12:2853–2854

    CAS  PubMed  Google Scholar 

  7. Meyer WR, Castelbaum AJ, Somkuti S, et al.: Hydrosalpinges adversely affect markers of endometrial receptivity. Hum Reprod 1997;12:1393–1398

    CAS  PubMed  Google Scholar 

  8. Barmat LI, Nasti K, Yang X, Spandorfer S, Kowalik A, El-Roeiy A: Are cytokines and growth factors responsible for the detrimental effects of hydrosalpingeal fluid on pregnancy rates after in vitro fertilization-embryo transfer? Fertil Steril 1999;72:1110–1112

    CAS  PubMed  Google Scholar 

  9. Mukherjee T, Copperman AB, McCaffrey C, Cook CA, Bustillo M, Obasaju MF: Hydrosalpinx fluid has embryotoxic effects on murine embryogenesis: A case for prophylactic salpingectomy. Fertil Steril 1996;66:851–853

    CAS  PubMed  Google Scholar 

  10. Rawe VJ, Liu J, Shaffer S, Compton MG, Garcia JE, Katz E: Effect of human hydrosalpinx fluid on murine embryo development and implantation. Fertil Steril 1997; 68:668–670

    CAS  PubMed  Google Scholar 

  11. Sachdev R, Kemmann E, Bohrer MK, el-Danasouri I: Detrimental effect of hydrosalpinx fluid on the development and blastulation of mouse embryos in vitro. Fertil Steril 1997;68:531–533

    CAS  PubMed  Google Scholar 

  12. Roberts JE, Clarke HJ, Tulandi T, Tan SL: Effects of hydrosalpingeal fluid on murine embryo development and implantation. J Assist Reprod Genet 1999;16:421–424

    CAS  PubMed  Google Scholar 

  13. Beyler SA, James KP, Fritz MA, Meyer WR: Hydrosalpingeal fluid inhibits in-vitro embryonic development in a murine model. Hum Reprod 1997;12:2724–2728

    CAS  PubMed  Google Scholar 

  14. Bedaiwy MA, Goldberg JM, Falcone T, et al.: Relationship between oxidative stress and embryotoxicity of hydrosalpingeal fluid. Hum Reprod 2002;17:601–604

    PubMed  Google Scholar 

  15. Ng EH, Yeung WS, Ho PC: The presence of hydrosalpinx may not adversely affect the implantation and pregnancy rates in in vitro fertilization treatment. J Assist Reprod Genet 1997;14:508–512

    CAS  PubMed  Google Scholar 

  16. Pellicer A, Albert C, Mercader A, Bonilla-Musoles F, Remohi J, Simon C: The follicular and endocrine environment in women with endometriosis: local and systemic cytokine production. Fertil Steril 1998;70:425–431

    CAS  PubMed  Google Scholar 

  17. Pellicer A, Valbuena D, Bauset C, et al. The follicular endocrine environment in stimulated cycles of women with endometriosis: steroid levels and embryo quality. Fertil Steril 1998;69:1135–1141

    CAS  PubMed  Google Scholar 

  18. Chen HL, Marcinkiewicz JL, Sancho-Tello M, Hunt JS, Terranova PF: Tumor necrosis factor-alpha gene expression in mouse oocytes and follicular cells. Biol Reprod 1993;48:707–714

    CAS  PubMed  Google Scholar 

  19. Haimovici F, Anderson DJ: Effects of growth factors and growth factor-extracellular matrix interactions on mouse trophoblast outgrowth in vitro. Biol Reprod 1993;49:124–130

    CAS  PubMed  Google Scholar 

  20. Ray P, Ghosh SK, Zhang DH, Ray A: Repression of interleukin-6 gene expression by 17 beta-estradiol: Inhibition of the DNA-binding activity of the transcription factors NF- IL6 and NF-kappa B by the estrogen receptor. FEBS Lett 1997;409:79–85

    CAS  PubMed  Google Scholar 

  21. Gorospe WC, Hughes FM, Jr., Spangelo BL: Interleukin-6: effects on and production by rat granulosa cells in vitro. Endocrinology 1992;130:1750–1752

    CAS  PubMed  Google Scholar 

  22. Jacobs AL, Sehgal PB, Julian J, Carson DD: Secretion and hormonal regulation of interleukin-6 production by mouse uterine stromal and polarized epithelial cells cultured in vitro. Endocrinology 1992;131:1037–1046

    CAS  PubMed  Google Scholar 

  23. Akoum A, Lemay A, Paradis I, Rheault N, Maheux R: Secretion of interleukin-6 by human endometriotic cells and regulation by proinflammatory cytokines and sex steroids. Hum Reprod 1996;11:2269–2275

    CAS  PubMed  Google Scholar 

  24. Arici A, Tazuke SI, Attar E, Kliman HJ, Olive DL: Interleukin-8 concentration in peritoneal fluid of patients with endometriosis and modulation of interleukin-8 expression in human mesothelial cells. Mol Hum Reprod 1996;2:40–45

    CAS  PubMed  Google Scholar 

  25. McKenzie AN, Culpepper JA, de Waal Malefyt R, et al.: Interleukin 13, a T-cell-derived cytokine that regulates human monocyte and B-cell function. Proc Natl Acad Sci USA 1993;90:3735–3739

    CAS  PubMed  Google Scholar 

  26. Van Voorhis BJ, Dunn MS, Snyder GD, Weiner CP: Nitric oxide: an autocrine regulator of human granulosa-luteal cell steroidogenesis. Endocrinology 1994;135:1799–1806

    CAS  PubMed  Google Scholar 

  27. Vlad M, Walker D, Kennedy RC: Nuclei number in human embryos co-cultured with human ampullary cells. Hum Reprod 1996;11:1678–1686

    CAS  PubMed  Google Scholar 

  28. Liu LP, Chan ST, Ho PC, Yeung WS: Human oviductal cells produce high molecular weight factor(s) that improves the development of mouse embryo. Hum Reprod 1995;10:2781–2786

    CAS  PubMed  Google Scholar 

  29. Morgan K, Wiemer K, Steuerwald N, Hoffman D, Maxson W, Godke R: Use of videocinematography to assess morphological qualities of conventionally cultured and cocultured embryos. Hum Reprod 1995;10:2371–2376

    CAS  PubMed  Google Scholar 

  30. Spandorfer SD, Liu HC, Neuer A, Barmat LI, Davis O, Rosenwaks Z: The embryo toxicity of hydrosalpinx fluid is only apparent at high concentrations: an in vitro model that stimulates in vivo events. Fertil Steril 1999;71:619–626

    CAS  PubMed  Google Scholar 

  31. Koong MK, Jun JH, Song SJ, Lee HJ, Song IO, Kang IS: A second look at the embryotoxicity of hydrosalpingeal fluid: an in-vitro assessment in a murine model. Hum Reprod 1998;13:2852–2856

    CAS  PubMed  Google Scholar 

  32. Murray CA, Clarke HJ, Tulandi T, Tan SL: Inhibitory effect of human hydrosalpingeal fluid on mouse preimplantation embryonic development is significantly reduced by the addition of lactate. Hum Reprod 1997;12:2504–2507

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Department of Obstetrics &Gynecology, The Cleveland Clinic Foundation, 9500 Euclid Avenue, A81, Cleveland, Ohio, 44195

    Mohamed A. Bedaiwy, Tommaso Falcone, Jeffrey M. Goldberg, Marjan Attaran, Rakesh Sharma, Kurt Miller & Ashok Agarwal

  2. Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Urological Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, A81, Cleveland, Ohio, 44195

    Kurt Miller & Ashok Agarwal

  3. Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Department of Biostatistics and Epidemiology, The Cleveland Clinic Foundation, 9500 Euclid Avenue, A81, Cleveland, Ohio, 44195

    David R. Nelson

Authors
  1. Mohamed A. Bedaiwy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tommaso Falcone
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jeffrey M. Goldberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marjan Attaran
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rakesh Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kurt Miller
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. David R. Nelson
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ashok Agarwal
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mohamed A. Bedaiwy.

Additional information

Presented in part at the 17th Annual Meeting of the European Society of Human Reproduction and Embryology (ESHRE), Laussane, Switzerland, July 1-4 2001.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bedaiwy, M.A., Falcone, T., Goldberg, J.M. et al. Relationship between cytokines and the embryotoxicity of hydrosalpingeal fluid. J Assist Reprod Genet 22, 161–165 (2005). https://doi.org/10.1007/s10815-005-4913-7

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10815-005-4913-7

Keywords

Search

Navigation