Journal of Assisted Reproduction and Genetics

, Volume 21, Issue 11, pp 415–420 | Cite as

Comparative Study of Biological and Technological Characters in Three Generations of Silkworm Bombyx mori L. Ameiotic, Parthenogenetically Cloned Lines

  • H. Greiss
  • J. Vassilieva
  • N. Petkov
  • Z. Petkov


Purpose: Detect any deviation in biologic and technologic characters of eight ameiotic-parthenogenetically cloned lines of Bombyx mori L. from different origins from a normal sexually reproduced control line in three generations.

Methods: Comparative study of the three generations was conducted in SES, Vratza, unit of the National Center for Agrarian Sciences of Bulgaria after fixing all environmental rearing conditions.

Results: The ameiotic-parthen-clones displayed good parthenogenetic development, although total hatchability was significantly less than the sexually reproducing control populations. Survival rates between clones and control were not significantly different. All clones displayed significantly longer larval periods. Slight decline in second generation, and a steeper one in the third generation were observed for all eight cloned lines in cocoon weight, shell weight, and shell ratio and these differences were statistically significant. Cocoon yield was significantly lower than the control throughout the three generations.

Conclusions: Our parthen-cloning method has a high rate of success in comparison to other cloning methods, the cloned progeny populations although were weaker technologically (cocoon weight, shell weight, and shell ratio), the biological characters (parthenogenetic development and survival rate) were not compromised. Further study is needed to determine the thermal needs of the cloned embryos and metabolic rate of all stages.

Ameiotic Bombyx moricloning parthenogenesis 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Strunnikov VA, Lejenko SS, Stepanova NL: Silkworm Bombyx mori L. cloning. Genetics 1983; 19(1): 82–94Google Scholar
  2. 2.
    Astaurov BL: Artificial Silkworm Parthenogenesis. Moscow, AS USSR, 1940, pp 238–302Google Scholar
  3. 3.
    Astaurov BL: Selection choice for thermal artificial parthenogenesis development ability in silkworm. Genetics 1973; 9(9): 93–105Google Scholar
  4. 4.
    Strunnikov, V: Male generation receiving in slikworm Bombyx mori L., Reports of Academy of Sciences, Moscow, 1990, vol. 18, pp. 5–32.Google Scholar
  5. 5.
    Vasileva J, Tzenov P: Heterosis, depression and degrees of dominance at silkworm Bombyx. Mori L. characters pigmented and non-pigmented parthenogenetic eggs. Anim Sci 2003; 1/2: 103–105Google Scholar
  6. 6.
    Vasileva J, Tzenov P: Study on parthenogenetic development of some silkworm Bombyx mori L. F1 hybrids. Anim Sci 2003; 3/4: 128–131Google Scholar
  7. 7.
    Astaurov, B: Artificial partheogenesis in silkworm Bombyx mori L., Academy of Science Publishing, Moscow, pp 240.Google Scholar
  8. 8.
    Astaurov BL: Experimental alterations of the developmental cytogenetic mechanisms in the mulberry silkworm: Artificial parthenogenesis, polyploidy, gynogenesis and androgenesis. Adv Morphol 1967; 6: 199–257Google Scholar
  9. 9.
    Vereiskaya V: Meioses and changes in thermo activated silkworm Bombyx mori L ovarioles. Bull Moscow Soc Nat Defender 1975; 4: 31–50Google Scholar
  10. 10.
    Klymenko VV: Mechanism silkworm artificial parthenogenesis. I. Dependence between thermoactivation effect and speed of after activation cooling. Genetics XVI, 1980; 6: 1002– 1010Google Scholar
  11. 11.
    Strunnikov V: Genetic methods of sex selection in silkworm. Moscow, 1987, pp 327.Google Scholar
  12. 12.
    Vasivela J, Tzenov P: Study of influence of before and after activating the ovariole silkworm eggs preservation at thermal ameiotic development ability. In Scientific Conference “Sciences for Life at New Millennium,” Agricultural University, Plovdiv, Bulgaria, 2000, pp 76–82.Google Scholar
  13. 13.
    Murakami A: Genetic studies on parthenogenesis in B. mori L. thermal activation and its significance. Annu Rep Natl Inst Genet Jpn 1985; 36: 48–49Google Scholar
  14. 14.
    Vasileva J, Mladenov G: Investigation on possibilities for creation of ameiotic parthenogenetic silkworm individuals from Super1xHessa2. Anim Sci 1998; Suppl.: 79–81Google Scholar
  15. 15.
    Klymenko VV: Parthenogenesis and cloning in the silkworm, B.mori L. Problems and prospects. J. Insect Biotech Seric 2001; 70: 155–165Google Scholar
  16. 16.
    Ravindra S, Raghavendra RD, Kariappa, BK, Prabhati S, Premalatha V: Studies on parthenogenetic development, hybrid vigor and cocoon shape variability in bivoltine F1 hybrids of the silkworm, B.mori L. Int J Ind Entomol 2002; 5(1): 59–65Google Scholar
  17. 17.
    Strunnikov VA, Gubanov EA, Zvyaginceva TV, Strunnikova LV: Silkworm eggs activation at different combination of thermal effects. Acad Sci Rep 1996; 348(6): 849–850Google Scholar
  18. 18.
    Petkov M, Penkov I: Sericulture Reference Book. Sofia, Bulgaria, Zemizdat, 1980, pp 200Google Scholar
  19. 19.
    Lim S, Kim Y, Lee S, Rhee I, Lim J, Lim B: Sericulture Training Manual. Rome, FAO, 1990, pp 200Google Scholar
  20. 20.
    Greiss H, Sohn HR, Lim JS: Study on the effect of different temperatures on the main productive characters of the silkworm larvae Bombyx mori L. under the Egyptian conditions. Agric Res Bull Kyungpook Natl Univ 2000; 18(12): 43–51Google Scholar
  21. 21.
    Kipriotis E, Petkov N, Tzenov P, Grekov D: Practical Sericulture. National Agricultural Research Foundation. Komotini, Greece, 1999, pp 130.Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2004

Authors and Affiliations

  • H. Greiss
    • 1
  • J. Vassilieva
    • 1
  • N. Petkov
    • 1
  • Z. Petkov
    • 1
  1. 1.Sericulture Experiment StationBulgaria

Personalised recommendations