Journal of Applied Spectroscopy

, Volume 83, Issue 2, pp 234–239 | Cite as

Spectrophotometric Determination of the Characteristics of Stromal and Parenchymal Components of Colon Tumors

  • I. G. Motevich
  • N. D. Strekal
  • A. V. Shulha
  • S. A. Maskevich

We consider the dependence of the spectral properties of eosin and hematoxylin (dyes routinely used in histology as contrast agents) on their localization in biological tissues with different levels of pathology: benign and malignant neoplasms and sigmoid colonic crypts. We have analyzed the fluorescent images and fluorescence spectra of the parenchyma and stromal elements. We have established that on going from physiologically normal cells to tumor cells, the contribution to the absorption cross section of histologic sections due to hematoxylin increases. In pathologically altered cells in a colonic crypt, we observe a hypsochromic effect in the fluorescence spectra of the samples with appreciable quenching of the fluorescence, while in the model systems the reverse effect occurs: a shift of the fluorescence maximum toward the red region. We discuss the influence on the indicated effects from local pH and the polarity of the dye environment in the model systems and histologic sections. As the systems modeling the polarity and acidity of the biological media, we use aqueous solutions of the dyes with different pH values and synthetic polyelectrolytes.

Key words

fluorescence spectrum eosin hematoxylin tumor cells stroma 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    D. B. Agus, J. F. Alexander, W. Arap, S. Ashili, J. E. Aslan, R. H. Austin, V. Backman, K. J. Bethel, R. Bonneau, W. C. Chen, C. Chen-Tanyolac, N. C. Choi, S. A. Curley, M. Dallas, D. Damania, P. C. Davies, P. Decuzzi, L. Dickinson, L. Estevez-Salmeron, V. Estrella, M. Ferrari, C. Fischbach, J. Foo, S. I. Fraley, C. Frantz, A. Fuhrmann, P. Gascard, R. A. Gatenby, Y. Geng, S. Gerecht, R. J. Gillies, B. Godin, W. M. Grady, A. Greenfi eld, C. Hemphill, B. L. Hempstead, A. Hielscher, W. D. Hillis, E. C. Holland, A. Ibrahim-Hashim, T. Jacks, R. H. Johnson, A. Joo, J. E. Katz, L. Kelbauskas, C. Kesselman, M. R. King, K. Konstantopoulos, C. M. Kraning-Rush, P. Kuhn, K. Kung, B. Kwee, J. N. Lakins, G. Lambert, D. Liao, J. D. Licht, J. T. Liphardt, L. Liu, M. C. Lloyd, A. Lyubimova, P. Mallick, J. Marko, O. J. McCarty, D. R. Meldrum, F. Michor, S. M. Mumenthaler, V. Nandakumar, T. V. O’Halloran, S. Oh, R. Pasqualini, M. J. Paszek, K. G. Philips, C. S. Poultney, K. Rana, C. A. Reinhart-King, R. Ros, G. L. Semenza, P. Senechal, M. L. Shuler, S. Srinivasan, J. R. Staunton, Y. Stypula, H. Subramanian, T. D. Tlsty, G. W. Tormoen, Y. Tseng, A. van Oudenaarden, S. S. Verbridge, J. C. Wan, V. M. Weaver, J. Widom, C. Will, D. Wirtz, J. Wojtkowiak, and P. H. Wu, Sci. Rep., 3, 1449 (2013).Google Scholar
  2. 2.
    S. D. Lung, Clin. Cancer Res., 8, No. 1, 1–3 (2002).Google Scholar
  3. 3.
    R. Todd and D. T. Wong, Histology and Histopathology, No. 14, 491–500 (1999).Google Scholar
  4. 4.
    E. V. Novichkov and A. A. Votintsev, Arkhiv Patologii, 67, No. 4, 29–31 (2005).Google Scholar
  5. 5.
    N. A. Bhowmick, E. G. Neilson, and H. L. Moses, Nature, 432, 332–337 (2004).ADSCrossRefGoogle Scholar
  6. 6.
    M. J. Bissell and D. Radisky, Nat. Rev. Cancer, 1, 46–54 (2001).CrossRefGoogle Scholar
  7. 7.
    H. Dolznig, C. Rupp, C. Puri, C. Haslinger, N. Schweifer, E. Wieser, D. Kerjaschki, and P. Garin-Chesa, Am. J. Pathol., 179, 487–501 (2011).CrossRefGoogle Scholar
  8. 8.
    P. Zigrino, S. Löffek, and C. Mauch, Biochim., 87, No. 5, 321–328 (2005).CrossRefGoogle Scholar
  9. 9.
    C. N. Baxevanis, G. V. Dedoussis, N. G. Papadopoulos, I. Missitzis, G. P. Stathopoulos, and M. Papamichail, Cancer, 74, No. 4, 1275–1282 (1994).CrossRefGoogle Scholar
  10. 10.
    G. A. Merkulov, A Course in Pathohistological Technique [in Russian], Meditsina, St. Petersburg (1969).Google Scholar
  11. 11.
    C. Bettinger and H. W. Zimmermann, Histochemistry, 96, No. 3, 215–228 (1991).CrossRefGoogle Scholar
  12. 12.
    Y. S. Heo and H. J. Song, Ann. Dermatol., 23, No. 1, 44–52 (2011).MathSciNetCrossRefGoogle Scholar
  13. 13.
    A. Tuer, D. Tokarz, N. Prent, R. Cisek, J. Alami, D. J. Dumont, L. Bakueva, J. Rowlands, and V. Barzda, J. Biomed. Opt., 15, No. 2 (2010).Google Scholar
  14. 14.
    T. J. Deerinck, M. E. Martone, V. Lev-Ram, D. P. Green, R. Y. Tsien, D. L. Spector, S. Huang, and M. H. Ellisman, J. Cell Biol., 126, No. 4, 901–910 (1994).CrossRefGoogle Scholar
  15. 15.
    E. M. Bukharova and I. M. Vlasova, Proceedings of International Conference "Lomonosov-2007", 11–14 Aril 2007, Moscow, M. V. Lomonosov MGU (2007), pp. 148–149.Google Scholar
  16. 16.
    S. Acharya and B. Rebery, Arab. J. Chem., 2, 7–12 (2009).CrossRefGoogle Scholar
  17. 17.
    M. H. Bar-Cellos-Hoff and S. A. Ravani, Cancer Res., 60, 1254–1260 (2000).Google Scholar
  18. 18.
    C. Billottet and J. Jouanneau, Bull. Cancer, 95, No. 1, 51–56 (2008).Google Scholar
  19. 19.
    M. V. Maffini, A. M. Soto, J. M. Calabro, A. A. Ucci, and C. Sonnenschein, J. Cell Sci., 117, 1495–1502 (2004).Google Scholar
  20. 20.
    A. Sadlonova, Z. Novak, M. R. Johnson, D. B. Bowe, S. R. Gault, G. P. Page, J. V. Thottassery, D. R. Welch, and A. R. Frost, Breast Cancer Res., 7, No. 1, 46–59 (2005).CrossRefGoogle Scholar
  21. 21.
    R. F. Hwang, T. Moore, T. Arumugam, V. Ramachandran, K. D. Amos, A. Rivera, B. Ji, D. B. Evans, and C. D. Logsdon, Cancer Res., 68, No. 3, 918–926 (2008).CrossRefGoogle Scholar
  22. 22.
    V. S. Schellerer, M. Langheinrich, W. Hohenberger, R. S. Croner, S. Merkel, T. T. Rau, M. Stürzl, and E. Naschberger, Oncol. Rep., 31, No. 1, 255–261 (2014).Google Scholar
  23. 23.
    G. A. Frank, A. A. Ivanov, M. A. Pal’tsev, Yu. Yu. Andreeva, A. V. Kuznetsova, B. Ya. Alekseev, T. I. Danilova, and O. P. Gladkikh, Arkhiv Patologii, No. 3, 27–30 (2004).Google Scholar
  24. 24.
    N. Cheng, A. Chytil, Y. Shyr, A. Joly, and H. L. Moses, Mol. Cancer Res., 6, No. 10, 1521–1533 (2008).CrossRefGoogle Scholar
  25. 25.
    Q. Yao, X. Qu, Q. Yang, M. Wei, and B. Kong, Oncol. Rep., 22, No. 3, 541–548 (2009).Google Scholar
  26. 26.
    M. Klink, K. Jastrzembska, M. Nowak, K. Bednarska, M. Szpakowski, K. Szyllo, and Z. Sulowska, Scand. J. Immunol., 68, No. 3, 328–336 (2008).CrossRefGoogle Scholar
  27. 27.
    P. Allavena, A. Sica, G. Solinas, C. Porta, and A. Mantovani, Crit. Rev. Oncol. Hematol., 66, No. 1, 1–9 (2008).CrossRefGoogle Scholar
  28. 28.
    I. G. Motevich, N. D. Strekal, N. M. Papko, M. I. Glebovich, A. V. Shulha, and S. A. Maskevich, Zh. Prikl. Spektrosk., 82, No. 1, 103–108 (2015) [I. G. Motevich, N. D. Strekal, N. M. Papko, M. I. Glebovich, A. V. Shulha, and S. A. Maskevich, J. Appl. Spectrosc., 82, 98–103 (2015) (English translation)].Google Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • I. G. Motevich
    • 1
  • N. D. Strekal
    • 1
  • A. V. Shulha
    • 2
  • S. A. Maskevich
    • 1
  1. 1.Yanka Kupala Grodno State UniversityGrodnoBelarus
  2. 2.Grodno State Medical UniversityGrodnoBelarus

Personalised recommendations