Journal of Applied Spectroscopy

, Volume 82, Issue 6, pp 1060–1063 | Cite as

UV Spectrophotometric Method for Estimation of Polypeptide-K in Bulk and Tablet Dosage Forms

  • P. Kaur
  • S. Kumar Singh
  • M. Gulati
  • Y. Vaidya

An analytical method for estimation of polypeptide-k using UV spectrophotometry has been developed and validated for bulk as well as tablet dosage form. The developed method was validated for linearity, precision, accuracy, specificity, robustness, detection, and quantitation limits. The method has shown good linearity over the range from 100.0 to 300.0 μg/ml with a correlation coefficient of 0.9943. The percentage recovery of 99.88% showed that the method was highly accurate. The precision demonstrated relative standard deviation of less than 2.0%. The LOD and LOQ of the method were found to be 4.4 and 13.33, respectively. The study established that the proposed method is reliable, specific, reproducible, and cost-effective for the determination of polypeptide-k.


polypeptide-k diabetes mellitus UV spectrometry 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • P. Kaur
    • 1
  • S. Kumar Singh
    • 1
  • M. Gulati
    • 1
  • Y. Vaidya
    • 1
  1. 1.School of Pharmaceutical SciencesLovely Professional UniversityPunjabIndia

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