Honey was found to quench the intrinsic fluorescence of bovine serum albumin (BSA) in a concentration dependent manner, showing complete quenching in the presence of 5% (w/v) honey. Increasing the protein concentration up to 5.0 μM did not lead to the recovery of the protein fluorescence. Urea denaturation of BSA, which otherwise shows a two-step, three-state transition, using intrinsic fluorescence of the protein as the probe failed to produce any result in the presence of 5% (w/v) honey. Thus, intrinsic fluorescence cannot be used as a spectral probe for protein denaturation studies in the presence of honey.
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Published in Zhurnal Prikladnoi Spektroskopii, Vol. 82, No. 5, pp. 788–791, September–October, 2015.
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Wong, Y.H., Kadir, H.A. & Tayyab, S. Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey. J Appl Spectrosc 82, 845–848 (2015). https://doi.org/10.1007/s10812-015-0191-z
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DOI: https://doi.org/10.1007/s10812-015-0191-z