Journal of Applied Spectroscopy

, Volume 81, Issue 3, pp 360–364 | Cite as

Spectral Properties of the Anionic Form of the Molecular Probe Fet

  • V. I. Tomin
  • R. Jaworski

The relationship of the fluorescence spectral characteristics of the anionic form of the molecular probe 4′-(diethylamino)-3-hydroxyflavone (FET) to intramolecular proton transfer in a weakly polar solvent (CH2Cl2) was studied. The fluorescence spectrum of this form had a broad featureless band in the area between the shortwavelength and long-wavelength fluorescence bands of FET. Its excitation spectrum had strong UV maxima (350 and 305 nm). This fluorescence disappeared completely if the solution was cooled to 1 °C. The deprotonation mechanism of neutral FET molecules was discussed.


fluorescence proton transfer anionic form probe 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Institute of Physics, Pomeranian University in SlupskSlupskPoland

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