The relationship of the fluorescence spectral characteristics of the anionic form of the molecular probe 4′-(diethylamino)-3-hydroxyflavone (FET) to intramolecular proton transfer in a weakly polar solvent (CH2Cl2) was studied. The fluorescence spectrum of this form had a broad featureless band in the area between the shortwavelength and long-wavelength fluorescence bands of FET. Its excitation spectrum had strong UV maxima (350 and 305 nm). This fluorescence disappeared completely if the solution was cooled to 1 °C. The deprotonation mechanism of neutral FET molecules was discussed.
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 81, No. 3, pp. 338–343, May–June, 2014.
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Tomin, V.I., Jaworski, R. Spectral Properties of the Anionic Form of the Molecular Probe Fet. J Appl Spectrosc 81, 360–364 (2014). https://doi.org/10.1007/s10812-014-9937-2
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DOI: https://doi.org/10.1007/s10812-014-9937-2