Spectroscopic Study on the Interaction of 4-dimethylaminochalcones with Phospholipids
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The ultraviolet-visible and fluorescence spectroscopic properties of 4′-dimethylaminochalcone (1a) and its cyclic analogs 2a–4a have been studied in the presence of phospholipid vesicles (i.e., egg yolk lecithin and dipalmitoylpho sphatidylcholine), bovine serum albumin (BSA), and lipoprotein particles (i.e., bovine serum albumin plus egg yolk lecithin). The spectral results showed that compounds 1a–4a formed hydrophobic interactions with the phospholipids, lipoproteins, and BSA at the polar/nonpolar interface. Compounds 3a and 4a exhibited the strongest hydrophobic interactions of all of the compounds tested towards the phospholipids. Compound 2a gave the best fluorescent fluorophore indicating interactions with the lipids, lipoproteins, and proteins. Fluorescent microscopic imaging of breast cancer cells treated with compounds 1a–4a revealed that they could be used to stain all of the cellular components and destroy the nuclear structure. Compounds 1a–4a were found to be concentrated predominantly on the surfaces of the liposomes and lipoproteins.
Keywordsdimethylaminochalcone fluorescence spectroscopy phospholipids lipoproteins breast cancer cells
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