Journal of Applied Spectroscopy

, Volume 73, Issue 1, pp 115–122 | Cite as

Effect of intravenous laser irradiation on the molecular structure of blood and blood components

  • G. A. Zalesskaya
  • E. G. Sambor
  • A. V. Kuchinskii


We present the results of a spectral study of the effect of low-intensity laser radiation on the molecular structure of blood and blood components. Analysis of the Fourier transform IR absorption spectra of blood confirmed the changes we observed previously in the oxygen transport characteristics of blood with intravenous exposure to the emission from a He-Ne laser. We show that structural and conformational changes in the hemoglobin tetramer, initiated by laser-induced photoreactions between Hb and oxygen, lead to characteristic changes in the shape and intensity of the IR bands for NH stretching vibrations, and also the amide I and amide II absorption bands. In the IR spectra of irradiated blood samples, we note increased absorption in the bands for stretching vibrations of the phosphate groups (945–1280 cm−1), which is evidence for an increase in the nucleic acid content (DNA, RNA). In the spectra of plasma and erythrocytes prepared from irradiated blood, there are no changes in this region of the IR spectrum. At the same time, in the IR spectra of samples of irradiated plasma, the intensity of the bands for stretching vibrations of the CH2 groups increases substantially.

Key words

blood plasma erythrocytes IR spectroscopy intravenous laser irradiation of blood helium-neon laser hemoglobin DNA 


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • G. A. Zalesskaya
    • 1
  • E. G. Sambor
    • 1
  • A. V. Kuchinskii
    • 1
  1. 1.Institute of Molecular and Atomic PhysicsNational Academy of Sciences of BelarusMinsk

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