Abstract
We present the results of a spectral study of the effect of low-intensity laser radiation on the molecular structure of blood and blood components. Analysis of the Fourier transform IR absorption spectra of blood confirmed the changes we observed previously in the oxygen transport characteristics of blood with intravenous exposure to the emission from a He-Ne laser. We show that structural and conformational changes in the hemoglobin tetramer, initiated by laser-induced photoreactions between Hb and oxygen, lead to characteristic changes in the shape and intensity of the IR bands for NH stretching vibrations, and also the amide I and amide II absorption bands. In the IR spectra of irradiated blood samples, we note increased absorption in the bands for stretching vibrations of the phosphate groups (945–1280 cm−1), which is evidence for an increase in the nucleic acid content (DNA, RNA). In the spectra of plasma and erythrocytes prepared from irradiated blood, there are no changes in this region of the IR spectrum. At the same time, in the IR spectra of samples of irradiated plasma, the intensity of the bands for stretching vibrations of the CH2 groups increases substantially.
Similar content being viewed by others
References
W. Yu., M. Mogowan, L. Naim et al., Las. Surg. Med., 8, 8–10 (1996).
J. Jurner and L. Hode, Low Level Laser Therapy: Clinical Practice and Scientific Background, Prima Books, Grangesberg (1999).
S. V. Moskvin and G. A. Azizov, Intravenous Laser Irradiation of Blood [in Russian], Tekhnika, Moscow (2003).
T. I. Karu, in: Biomedical Photonics Handbook [Tuan Vo-Dinh, ed.], CRC Press, Boca Raton, Florida (2003), pp. 48-1–48-2.
L. J. Bellamy, New Data on Infrared Spectra of Complex Molecules [Russian translation], Mir, Moscow (1971).
G. A. Zalesskaya and E. G. Sambor, Zh. Prikl. Spektr., 72, 230–235 (2005).
O. A. Vladimirov, G. I. Klebanov, G. G. Borisenko, and A. N. Osipov, Biofizika, 49, 339–350 (2004).
M. F. Perutz, J. E. Ladner, S. R. Simon, and C. Ho, Biochemistry, 13, 2163–2173 (1974).
Yu. I. Musienko and N. I. Nechipurenko, in: Current Problems in Neurology and Neurosurgery [in Russian], Minsk (2005), Vol. 7, pp. 89–95.
T. Karu, J. Photochem. Photobiol. B: Biol., 49, 1–17 (1999).
N. D. Devyatkov, S. M. Zubkova, I. B. Laprun, and N. S. Makeeva, Usp. Sovr. Biologii, 103, 31–43 (1987).
L. N. Kartusova, “Effect of radiation from a helium-neon laser on the physicochemical properties of blood,” Author’s Abstract, Dissertation in competition for the academic degree of Candidate of Biological Sciences, Moscow (1996).
S. D. Zakharov and A. V. Ivanov, Kvant. Élektron., 29, 192–214 (1999).
C. P. Schultz, Kan-Zhi Liu, J. B. Johnston, and H. H. Mantsch, J. Mol. Struct., 408/409, 253–256 (1997).
G. A. Zalesskaya, N. I. Nechipurenko, E. G. Sambor, and Yu. I. Musienko, Proceedings of the International Conference on Optical Laser Technology in Biology and Medicine [in Russian], Minsk (2004), Vol. 1, pp. 145–152.
G. A. Zalesskaya, E. G. Sambor, and N. I. Nechipurenko, Technical Digest ICONO/LAT, St. Petersburg, Russia (2005), ISF4.
T. V. Chichuk, I. A. Strashkevich, and G. I. Klebanov, Vestn. Ros. Akad. Med. Nauk, 2, 27–31 (1999).
D. A. Rogatkin and V. V. Chernyi, “Low-level laser therapy. Physics of the mechanism of action and practical experience,” http://bsfp.iszf.irk.ru/bsfp1999/bsff2/bb05bn5.php (1999).
J. Mordehai, J. Ramesh, M. Huleihel et al., “Health status prediction using FTIR microspectroscopy of blood components and cluster analysis,” http://www.bgu.ac.il/∼shaulm/papers/healthstat.pdf (2003).
Author information
Authors and Affiliations
Corresponding author
Additional information
__________
Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 73, No. 1, pp. 106–112, January–February, 2006.
Rights and permissions
About this article
Cite this article
Zalesskaya, G.A., Sambor, E.G. & Kuchinskii, A.V. Effect of intravenous laser irradiation on the molecular structure of blood and blood components. J Appl Spectrosc 73, 115–122 (2006). https://doi.org/10.1007/s10812-006-0045-9
Received:
Issue Date:
DOI: https://doi.org/10.1007/s10812-006-0045-9