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Influence of chitosan-based carbon dots added in MgAC-containing culture medium on green alga Tetraselmis sp.

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Abstract

The aim of this study was to evaluate the growth-promoting effects of the combination of magnesium aminoclay (MgAC) and amino-functionalized carbon dots (N(CD)s) on Tetraselmis sp. cultures with an emphasis on N(CD)s. To that end, simply sol-gel-synthesized MgAC and one-pot carbonization-synthesized N(CD)s were utilized. The as-prepared N(CD)s possessed the expected amino-functional groups, exhibited strong blue emission with bright luminescence, and showed a quantum yield (QY) of 5.69%. The maximum specific growth rate and biomass productivity were achieved with the combination of 0.1 g L−1 MgAC and 40 μg mL−1 N(CD)s, which afforded improvements in lipid and protein accumulation relative to the control, whereas carbohydrate accumulation slightly decreased. At low concentrations (0.1 g L−1), MgAC demonstrated growth stimulation. The N(CD) addition to the MgAC-containing medium further supported the Tetraselmis sp. cultures in counteracting the effects of UV radiation and in improving photosynthesis activity. In these ways, increased growth and biomass productivity under UV irradiation and enhanced pigment contents under the light-irradiance condition were obtained. Therefore, use of the MgAC-N(CD) combination as a culture-medium supplement is a promising method for microalgae biorefinery applications.

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Funding

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF2017R1D1A1A09000642), and this research was supported by Basic Science Research Capacity Enhancement Project through Korea Basic Science Institute (National research Facilities and Equipment Center) grant funded by the Ministry of Education (2019R1A6C1010016), and this work was supported by the Gachon University research fund of 2019 (GCU-2019-0818).

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Authors and Affiliations

  1. VNUK Institute for Research and Executive Education, The University of Danang, 158A Le Loi Street, Hai Chau District, Danang, Vietnam

    Minh Kim Nguyen

  2. Department of BioNano Technology, Gachon University, 1342 Seongnamdaero, Sujeong-gu, Seongnam-si, 13120, Gyeonggi-do, Republic of Korea

    Minh Kim Nguyen, Ju-Young Moon, Vinh Van Tran & Young-Chul Lee

  3. MCK Biotech Co. Ltd., #2509B, Global Venture Bld, Gyeongbuk Technopark, Gyeongsan, 38542, Republic of Korea

    Mi-Kyung Kim

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Contributions

Study conception and design, data acquisition, analysis and interpretation (M.K.N.); drafting (M.K.N.); critical revision for important content (M.K.N.; M.-K.K.; J.-Y.M.; V.V.T.; Y.-C.L.); final approval of manuscript (M.K.N.; M.-K.K.; J.-Y.M.; V.V.T.; Y.-C.L.).

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Correspondence to Young-Chul Lee.

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Nguyen, M.K., Kim, MK., Moon, JY. et al. Influence of chitosan-based carbon dots added in MgAC-containing culture medium on green alga Tetraselmis sp.. J Appl Phycol 33, 765–775 (2021). https://doi.org/10.1007/s10811-021-02368-5

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