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Evaluation of drying methods on nutritional constituents and antioxidant activities of Chlorella vulgaris cultivated in an outdoor open raceway pond

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Abstract

Chlorella vulgaris is known for its protein, growth factor, and nutritional constituents. Chlorella vulgaris was cultivated in a 1000-L outdoor open raceway pond with a maximum volumetric productivity of 130 mg L-1 day-1. The harvested biomass was dried through different methods, viz., sun drying (30 °C), oven drying (60 °C), lyophilization (−110 °C), drum drying (120 °C), and spray drying (100–150 °C). The effect of the drying method on proximate composition, pigments (chlorophyll, carotenoids), bioactive compounds (total phenolic content, flavonoid content), vitamin B12, antioxidant properties (ferric reducing antioxidant power, DPPH, and total antioxidant activity), and the color quality of C. vulgaris biomass was evaluated. Surface characterization by scanning electron microscopy (SEM) and functional group characterization through Fourier transform infrared spectroscopy were also performed. The biomass dried through lyophilization and sun drying retained maximum bioactive compounds and antioxidant activities. In contrast, drum drying resulted in a loss of nutrients, viz., protein (up to 44%), lipid (up to 41%), vitamin B12 (up to 40%), total phenolic content (> 50%), total flavonoid content (> 50%), and antioxidant activity (> 50%). Oven drying led to a loss of 30% in total flavonoid content and 17% in ferric reducing antioxidant power. SEM showed the destruction of cell wall integrity in the drum-dried sample and porous structure in the spray-dried sample. This study suggests that drying methods affect the nutrients and bioactive compounds of C. vulgaris biomass, and therefore a drying method should be selected carefully depending on the end use of the biomass.

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Acknowledgments

The authors thank the Director, CSIR-Central Food Technological Research Institute, Mysore, India, for providing facilities. CKMB acknowledges the Council of Scientific and Industrial Research, Government of India, for the award of SRF (31/005(0543)/2017-EMR-I). The authors also thank DBT, Govt. of India, New Delhi, India, for funding (Grant Number BT/PR6552/PBD/26/360/2012).

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Authors and Affiliations

  1. Plant Cell Biotechnology Department, CSIR–Central Food Technological Research Institute, Mysuru, Karnataka, 570020, India

    C. K. Madhubalaji, Sandeep N. Mudaliar, Vikas Singh Chauhan & R. Sarada

  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India

    C. K. Madhubalaji, Sandeep N. Mudaliar, Vikas Singh Chauhan & R. Sarada

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  1. C. K. Madhubalaji
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Madhubalaji, C.K., Mudaliar, S.N., Chauhan, V.S. et al. Evaluation of drying methods on nutritional constituents and antioxidant activities of Chlorella vulgaris cultivated in an outdoor open raceway pond. J Appl Phycol 33, 1419–1434 (2021). https://doi.org/10.1007/s10811-020-02355-2

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