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Transgenic eukaryotic microalgae as green factories: providing new ideas for the production of biologically active substances

  • 10th Asia-Pacific Conference on Algal Biotechnology
  • Published:
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Abstract

Eukaryotic microalgae are important primary producers in nature that play an important role in the energy cycle of nature. The eukaryotic microalgae are also regarded as potential bioactive substance producers. In recent years, with the aid of genetic engineering, eukaryotic microalgae have found a wider range of potential applications in medicine, food, health products, cosmetics, and environmental protection. This article reviews the state of the art of microalga genetic engineering from the aspects of gene transfer system, gene editing technology, and screening method, with examples of the enhancement of lipids, carotenoids, polysaccharide, and functional proteins. Potential application scenarios of microalga genetic engineering and their products in the field of food and medicine are also highlighted. Furthermore, strategies for protein expression optimization in eukaryotic microalgae are reviewed. The existing shortcomings of eukaryotic microalga genetic engineering are also analyzed and highlighted.

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Funding

This work was sponsored by National Key Research and Development Project of China 2019YFA0906300 and 2020YFA0907304, Natural Science Foundation of Shandong Province ZR2019ZD17, National Natural Science Foundation of China 31872608, Natural Science Foundation of Shanghai 18ZR1410100, Funding Project of the State Key Laboratory of Bioreactor Engineering, and Open Funding Project of the State Key Laboratory of Marine Resource Utilization in South China Sea (Hainan University) 2019002.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People’s Republic of China

    Qianwen Shi, Hui Wu, Haizhen Wu & Jianhua Fan

  2. Department of Applied Biology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People’s Republic of China

    Qianwen Shi, Cheng Chen, Wei Zhang, Ping Wu, Meng Sun, Haizhen Wu & Jianhua Fan

  3. State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, 58 Renmin Avenue, Haikou, 570228, Hainan Province, People’s Republic of China

    Pengcheng Fu

Authors
  1. Qianwen Shi
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  2. Cheng Chen
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  3. Wei Zhang
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  4. Ping Wu
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  5. Meng Sun
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  6. Hui Wu
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  7. Haizhen Wu
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  8. Pengcheng Fu
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  9. Jianhua Fan
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Contributions

Jianhua Fan: conceptualization, project administration, supervision. Pengcheng Fu: project administration, supervision. Qianwen Shi: investigation, writing—original draft, writing—review and editing. Cheng Chen: investigation, writing—original draft, writing—review and editing. Wei Zhang: writing—original draft. Ping Wu: writing—original draft. Hui Wu: supervision. Haizhen Wu: supervision.

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Correspondence to Pengcheng Fu or Jianhua Fan.

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Shi, Q., Chen, C., Zhang, W. et al. Transgenic eukaryotic microalgae as green factories: providing new ideas for the production of biologically active substances. J Appl Phycol 33, 705–728 (2021). https://doi.org/10.1007/s10811-020-02350-7

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  • DOI: https://doi.org/10.1007/s10811-020-02350-7

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