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Journal of Applied Phycology

, Volume 28, Issue 5, pp 2891–2902 | Cite as

Photosynthetic responses of ‘Neosiphonia sp. epiphyte-infected’ and healthy Kappaphycus alvarezii (Rhodophyta) to irradiance, salinity and pH variations

  • Iris Ann G. Borlongan
  • Ma. Rovilla J. Luhan
  • Philip Ian P. Padilla
  • Anicia Q. Hurtado
Article

Abstract

Understanding the physiological condition of seaweeds as influenced by biotic and abiotic stress is vital from the perspective of massive expansion and sustainability of seaweed-based industries. The photosynthetic responses of Neosiphonia sp. epiphyte-infected (INF) and healthy (HEA) Kappaphycus alvarezii under various combinations of irradiance, salinity and pH were studied using photosynthesis-irradiance (P-E) curves. Measurements of algal photosynthetic rates, expressed in terms of amount of oxygen production per fresh weight biomass per unit time (mg O2 g−1 FW h−1), were carried out using the light-dark bottle technique. Neosiphonia-infected K. alvarezii (INF) had lower photosynthetic rates than healthy ones (HEA). Similarities (p > 0.05) in light-saturated photosynthesis rates (P max) and significant differences (p < 0.05) in initial slope of curve (α) between INF and HEA K. alvarezii suggest that both samples are adapted to similar light conditions and differs only on photosynthetic efficiency. Low P max (0.7–2.0 mg O2 g−1 FW h−1) and high initial saturation irradiances (E k  = 90–519 μmol photons m−2 s−1) of INF seaweeds resulted to their low photosynthetic efficiency (α = 0.002–0.010). Such decline in α is attributed to the epiphyte, as Neosiphonia sp. covered almost the entire surface of K. alvarezii. An increase in chlorophyll-a (35–42.1 vs. 27.7–31.5 μg g−1 FW, HEA) and phycobilin (1.96–2.39 vs. 1.16–1.58 mg g−1 FW, HEA) contents was also observed in INF samples, suggesting acclimation to low-irradiance conditions, as a result of competition for light between the epiphyte and host. Both INF and HEA K. alvarezii also exhibited broad photosynthetic tolerance to short-term changes in irradiance, with no photoinhibition at the highest irradiance of 850 μmol photons m−2 s−1. K. alvarezii had a euryhaline photosynthetic response, with optimum salinity of 35 psu. Photosynthetic rates increased with decreasing pH, revealing K. alvarezii’s ability to modify its photosynthetic affinity for acidic seawater conditions; yet, their underlying mechanism of response to pH shifts still need to be further examined.

Keywords

Kappaphycus alvarezii Neosiphonia sp. Photosynthesis Seaweed cultivation Seaweed epiphytes Seaweed stress 

Notes

Acknowledgments

This research was funded in part by the University of the Philippines-Visayas (UPV) through the Office of the Vice-Chancellor for Research and Extension and Southeast Asian Fisheries Development Center Aquaculture Department. This research was the thesis submitted by the first author in fulfilment of her degree in Master of Science in Biology at UPV.

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Iris Ann G. Borlongan
    • 1
    • 4
  • Ma. Rovilla J. Luhan
    • 2
  • Philip Ian P. Padilla
    • 1
  • Anicia Q. Hurtado
    • 3
  1. 1.Division of Biological SciencesCollege of Arts and Sciences, University of the Philippines VisayasMiagaoPhilippines
  2. 2.Aquaculture DepartmentSoutheast Asian Fisheries Development CenterTigbauanPhilippines
  3. 3.Integrated Services for the Development of Aquaculture and Fisheries (ISDA) Inc.JaroPhilippines
  4. 4.Faculty of FisheriesKagoshima UniversityKagoshimaJapan

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