Journal of Applied Phycology

, Volume 28, Issue 5, pp 3043–3055 | Cite as

Genetic differences among varieties of Saccharina japonica in northern Japan as determined by AFLP and SSR analyses

  • Norishige Yotsukura
  • Takashi Maeda
  • Tsuyoshi Abe
  • Masahiro Nakaoka
  • Tadashi Kawai


The genetic diversity of Saccharina japonica inhabiting the Hokkaido coastline and the surrounding area was investigated by AFLP and SSR analyses. A STRUCTURE analysis based on the AFLP data identified two clusters in the species, although a few local populations with genetic structures different from that of the neighboring populations were detected. The two clusters could be identified even within each current variety. In the SSR analysis, genetic variation in nucleotide sequences in all samples was located in four DNA regions (MS10, MS11, MS16, and MS29), and it is thought that these regions can be used as markers to detect individual-level variation in S. japonica. The STRUCTURE analysis of the SSR data identified four clusters in the species. Since the geographical distribution of these four different clusters does not correspond exactly to the currently recognized varieties in this species, it will be necessary to re-evaluate the distribution of the varieties of S. japonica.


AFLP Distribution region Fragment analysis Genetic difference Saccharina japonica SSR 



The authors thank Prof. N Klochkova (Kamchatka State Technical University), Dr. D. Galanin (Sakhalin Research Institute of Fisheries and Oceanography), and Dr. S. Kirihara and Mr. Y. Fujikawa (Aomori Prefectural Industrial Technology Research Center) for collecting materials. This research was supported by a research project grant-in-aid for Scientific Research (25304010) from the Ministry of Education, Science, Sports, and Culture, Japan, and by the Environment Research and Technology Development Fund (S9) of the Ministry of the Environment, Japan.

Supplementary material

10811_2016_807_MOESM1_ESM.xlsx (46 kb)
Supplementary Table 1 Dice similarity coefficiency (S) of Saccharina japonica within a locality and between localities. (XLSX 45 kb)
10811_2016_807_MOESM2_ESM.xlsx (36 kb)
Supplementary Table 2 Pairsise (Gst) value of Saccharina japonica between localities. (XLSX 36 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Norishige Yotsukura
    • 1
  • Takashi Maeda
    • 2
  • Tsuyoshi Abe
    • 3
  • Masahiro Nakaoka
    • 4
  • Tadashi Kawai
    • 5
  1. 1.Field Science Center for Northern BiosphereHokkaido UniversityHokkaidoJapan
  2. 2.Hakodate Fisheries Experimental StationHokkaido Research OrganizationHokkaidoJapan
  3. 3.The Hokkaido University MuseumHokkaidoJapan
  4. 4.Akkeshi Marine StationHokkaido UniversityHokkaidoJapan
  5. 5.Wakkanai Fisheries Experimental StationHokkaido Research OrganizationHokkaidoJapan

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