Journal of Applied Phycology

, Volume 28, Issue 4, pp 2323–2332 | Cite as

Cell surface characterization of some oleaginous green algae

  • Ling Xia
  • Hongqiang Li
  • Shaoxian Song


This study reports a comprehensive set of experimentally measured surface properties of six oleaginous green microalgae. The results showed that the hydrophobic character of the six microalgae, determined by the contact angle method, was more accurate than by using the microbial adhesion to solvents (MATS) method. However, they both indicated that all studied microalgae presented an electron donor parameter. Due to the abundant surface carboxyl, phosphoryl, and hydroxyl groups, all microalgae presented a negatively charged surface. Monoraphidium dybowoskii XJ-377 was the most hydrophobic strain with a negative ΔG coh and the lowest surface free energy. Kirchneriella dianae XJ-93 had the lowest total surface functional group concentration and the lowest surface area, which can lower the harvesting cost. Overall, cellular surface properties should be evaluated and considered in oleaginous microalgal screening and identification in addition to the biomass and oil production normally considered.


Microalgae Cell surface property Hydrophobicity Surface free energy Lipid content 



This work was financially supported by the China Postdoctoral Science Foundation (2015M580671) and the National Natural Science Foundation of China under the project no. 51474167.


  1. Ahimou F, Paquot M, Jacques P, Thonart P, Paul GR (2001) Influence of electrical properties on the evaluation of the surface hydrophobicity of Bacillus subtilis. J Microbiol Meth 45:119–126CrossRefGoogle Scholar
  2. Amaro HM, Guedes AC, Malcata FX (2011) Advances and perspectives in using microalgae to produce biodiesel. Appl Energ 88:3402–3410CrossRefGoogle Scholar
  3. Barros AI, Gonçalves AL, Simões M, Pires JCM (2015) Harvesting techniques applied to microalgae: a review. Renew Sust Energy Rev 41:1489–1500CrossRefGoogle Scholar
  4. Bellon-Fontaine MN, Rault J, Van Oss C (1996) Microbial adhesion to solvents: a novel method to determine the electron-donor/electron-acceptor or Lewis acid-base properties of microbial cells. Colloids Surf B 7:47–53CrossRefGoogle Scholar
  5. Bligh E, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Phys 37:911–917CrossRefGoogle Scholar
  6. Busscher HJ, Weerkamp AH, van der Mei HC, Van Pelt A, de Jong HP, Arends J (1984) Measurement of the surface free energy of bacterial cell surfaces and its relevance for adhesion. Appl Environ Microbiol 48:980–983PubMedPubMedCentralGoogle Scholar
  7. Choi HJ, Lee SM (2015) Heavy metal removal from acid mine drainage by calcined eggshell and microalgae hybrid system. Environ Sci Pollut Res Int 22:13404–13411CrossRefPubMedGoogle Scholar
  8. Coward T, Lee JGM, Caldwell GS (2014) Harvesting microalgae by CTAB-aided foam flotation increases lipid recovery and improves fatty acid methyl ester characteristics. Biomass Bioenergy 67:354–362CrossRefGoogle Scholar
  9. Díaz-Santos E, Vila M, de la Vega M, León R, Vigara J (2015) Study of bioflocculation induced by Saccharomyces bayanus var. uvarum and flocculating protein factors in microalgae. Algal Res 8:23–29CrossRefGoogle Scholar
  10. Dixit S, Singh DP (2014) An evaluation of phycoremediation potential of cyanobacterium Nostoc muscorum: characterization of heavy metal removal efficiency. J Appl Phycol 26:1331–1342CrossRefGoogle Scholar
  11. Farooq W, Moon M, Ryu B, Suh WI, Shrivastav A, Park MS, Mishra SK, Yang JW (2015) Effect of harvesting methods on the reusability of water for cultivation of Chlorella vulgaris, its lipid productivity and biodiesel quality. Algal Res 8:1–7CrossRefGoogle Scholar
  12. Garg S, Li Y, Wang L, Schenk PM (2012) Flotation of marine microalgae: effect of algal hydrophobicity. Bioresour Technol 121:471–474CrossRefPubMedGoogle Scholar
  13. Gonçalves AL, Ferreira C, Loureiro JA, Pires JCM, Simões M (2015) Surface physicochemical properties of selected single and mixed cultures of microalgae and cyanobacteria and their relationship with sedimentation kinetics. Bioresour Bioproc 2:21CrossRefGoogle Scholar
  14. Griffiths MJ, Harrison ST (2009) Lipid productivity as a key characteristic for choosing algal species for biodiesel production. J Appl Phycol 21:493–507CrossRefGoogle Scholar
  15. Hadjoudja S, Deluchat V, Baudu M (2010) Cell surface characterisation of Microcystis aeruginosa and Chlorella vulgaris. J Colloid Interface Sci 342:293–299CrossRefPubMedGoogle Scholar
  16. Henderson R, Parsons SA, Jefferson B (2008) The impact of algal properties and pre-oxidation on solid-liquid separation of algae. Water Res 42:1827–1845CrossRefPubMedGoogle Scholar
  17. Henderson RK, Parsons SA, Jefferson B (2010) The impact of differing cell and algogenic organic matter (AOM) characteristics on the coagulation and flotation of algae. Water Res 44:3617–3624CrossRefPubMedGoogle Scholar
  18. Hermansson M (1999) The DLVO theory in microbial adhesion. Colloids Surf B 14:105–119CrossRefGoogle Scholar
  19. Hiemenz PC, Rajagopalan R (1997) Principles of colloid and surface chemistry, vol 14. CRC Press, New YorkGoogle Scholar
  20. Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54:621–639CrossRefPubMedGoogle Scholar
  21. Kiefer E, Sigg L, Schosseler P (1997) Chemical and spectroscopic characterization of algae surfaces. Environ Sci Technol 31:759–764CrossRefGoogle Scholar
  22. Liu T, Wang J, Hu Q, Cheng P, Ji B, Liu J, Chen Y, Zhang W, Chen X, Chen L (2013a) Attached cultivation technology of microalgae for efficient biomass feedstock production. Bioresource Technol 127:216–222CrossRefGoogle Scholar
  23. Liu CZ, Zheng S, Xu L, Wang F, Guo C (2013b) Algal oil extraction from wet biomass of Botryococcus braunii by 1, 2-dimethoxyethane. Appl Energ 102:971–974CrossRefGoogle Scholar
  24. Mayers JJ, Flynn KJ, Shields RJ (2013) Rapid determination of bulk microalgal biochemical composition by Fourier-Transform Infrared spectroscopy. Bioresource Technol 148:215–220CrossRefGoogle Scholar
  25. Mercer P, Armenta RE (2011) Developments in oil extraction from microalgae. Eur J Lipid Sci Technol 113:539–547CrossRefGoogle Scholar
  26. Naumann T, Çebi Z, Podola B, Melkonian M (2013) Growing microalgae as aquaculture feeds on twin-layers: a novel solid-state photobioreactor. J Appl Phycol 25:1413–1420CrossRefGoogle Scholar
  27. Ozkan A, Berberoglu H (2013) Physico-chemical surface properties of microalgae. Colloids Surf B 112:287–293CrossRefGoogle Scholar
  28. Rosenberg M, Gutnick D, Rosenberg E (1980) Adherence of bacteria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity. FEMS Microbiol Lett 9:29–33CrossRefGoogle Scholar
  29. Salim S, Bosma R, Vermuë MH, Wijffels RH (2011) Harvesting of microalgae by bio-flocculation. J Appl Phycol 23:849–855CrossRefPubMedGoogle Scholar
  30. Sheng GP, Yu HQ, Li XY (2010) Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review. Biotechnol Adv 28:882–894CrossRefPubMedGoogle Scholar
  31. Stanier R, Kunisawa R, Mandel M, Cohen-Bazire G (1971) Purification and properties of unicellular blue-green algae (order Chroococcales). Bact Rev 35:171PubMedPubMedCentralGoogle Scholar
  32. Van Den Hende S, Vervaeren H, Desmet S, Boon N (2011) Bioflocculation of microalgae and bacteria combined with flue gas to improve sewage treatment. New Biotech 29:23–31CrossRefGoogle Scholar
  33. Van Oss C (2008) The properties of water and their role in colloidal and biological systems. Academic, BuffaloGoogle Scholar
  34. Van Oss C, Chaudhury M, Good R (1987) Monopolar surfaces. Adv Colloid Interface Sci 28:35–64CrossRefPubMedGoogle Scholar
  35. Van Oss C, Good R, Chaudhury M (1988) Additive and nonadditive surface tension components and the interpretation of contact angles. Langmuir 4:884–891CrossRefGoogle Scholar
  36. Van Oss C, Ju L, Chaudhury M, Good R (1989) Estimation of the polar parameters of the surface tension of liquids by contact angle measurements on gels. J Colloid Interface Sci 128:313–319CrossRefGoogle Scholar
  37. Vandamme D, Foubert I, Meesschaert B, Muylaert K (2010) Flocculation of microalgae using cationic starch. J Appl Phycol 22:525–530CrossRefGoogle Scholar
  38. Williams D, Kuhn A, O’Bryon T, Konarik M, Huskey J (2011) Contact angle measurements using cell phone cameras to implement the Bikerman method. Galvanotechnik 102:1718–1725Google Scholar
  39. Yalcin S, Sezer S, Apak R (2012) Characterization and lead(II), cadmium(II), nickel(II) biosorption of dried marine brown macro algae Cystoseira barbata. Environ Sci Pollut Res Int 19:3118–3125CrossRefPubMedGoogle Scholar
  40. Yang J, Cao J, Xing G, Yuan H (2015) Lipid production combined with biosorption and bioaccumulation of cadmium, copper, manganese and zinc by oleaginous microalgae Chlorella minutissima UTEX2341. Bioresour Technol 175:537–544CrossRefPubMedGoogle Scholar
  41. Zhang X, Amendola P, Hewson JC, Sommerfeld M, Hu Q (2012) Influence of growth phase on harvesting of Chlorella zofingiensis by dissolved air flotation. Bioresour Technol 116:477–484CrossRefPubMedGoogle Scholar
  42. Zhang X, Jiang Z, Li M, Zhang X, Wang G, Chou A, Chen L, Yan H, Zuo YY (2014) Rapid spectrophotometric method for determining surface free energy of microalgal cells. Anal Chem 86:8751–8756CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.School of Resources and Environmental EngineeringWuhan University of TechnologyWuhanChina

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