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Journal of Applied Phycology

, Volume 28, Issue 4, pp 2233–2240 | Cite as

Cryopreservation of microalgae from desert environments of Qatar

  • Imen Saadaoui
  • Maryam Al Emadi
  • Touria Bounnit
  • Kira Schipper
  • Hareb Al Jabri
Article

Abstract

The Qatar University Culture Collection of Cyanobacteria and Microalgae (QUCCCM) is a unique resource containing a diverse collection of microalgae and cyanobacteria, isolated from the Qatar desert environment. In order to ensure maximum preservation of this resource, a number of cryopreservation techniques were applied to various strains, and the preservation effectiveness (cell viability and lipid productivity) was determined. The conditions tested were direct, passive, and freeze-cooling cryopreservation (technique), dimethyl sulfate and methanol (cryoprotectant), and 5 and 10 % cryoprotectant concentrations over storage durations of up to 1 year. It was shown that the cryopreservation regime is strain dependent, and strains belonging to the same genera can have different requirements. On the other hand, neutral lipid estimation, via Nile red fluorescence determination of pre- and post-cryopreserved microalgae isolates, confirmed that the lipid production is affected by the applied cryopreservation method.

Keywords

Microalgae Cryopreservation Cryoprotectant Long-term storage Neutral lipid 

Notes

Acknowledgments

This research was supported by Qatar Airways and the Qatar Scientific Technology Park (QSTP) and hosted by Qatar University. Very special regards go to the memory of Pr. Malcolm Potts who was always supportive and helpful.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Imen Saadaoui
    • 1
  • Maryam Al Emadi
    • 1
  • Touria Bounnit
    • 1
  • Kira Schipper
    • 1
  • Hareb Al Jabri
    • 1
  1. 1.Algal Technologies Program, Centre for Sustainable Development, College of Arts and SciencesQatar UniversityDohaQatar

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