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Journal of Applied Phycology

, Volume 27, Issue 6, pp 2367–2376 | Cite as

Antioxidant and anti-inflammatory activities of methanol extract and its fractions from the brown seaweed Spatoglossum schroederi

  • Sérvio Quesado Júnior
  • Victor Hugo Azevedo Carneiro
  • Thais Pontes Carvalho Fontenelle
  • Luciano de Sousa Chaves
  • Jacilane Ximenes Mesquita
  • Tarcísio Vieira de Brito
  • Rafael Siva Prudêncio
  • Jefferson Soares de Oliveira
  • Jand-Venes Rolim Medeiros
  • Karoline Saboia Aragão
  • Ronaldo de Albuquerque Ribeiro
  • André Luiz dos Reis Barbosa
  • Ana Lúcia Ponte Freitas
Article

Abstract

In the present study, the brown seaweed Spatoglossum schroederi was submitted to extraction procedures in order to obtain methanol (MET) extract and its hexane (HEX) and chloroform (CLR) fractions. All samples were evaluated for total phenolic and flavonoid contents as well as antioxidant and anti-inflammatory properties. The HEX fraction was the richest in total phenolic content, while CLR in total flavonoid content. MET, HEX, and CLR exhibited potent dose-dependent antioxidant activity by the total antioxidant capacity (TOAC) and reducing power methods and linear regression evidenced that the antioxidant activity of S. schroederi is due mainly to the flavonoid content. Following, MET extract and its fractions were evaluated for anti-inflammatory activity. Algae samples inhibited significantly the paw edema induced by carrageenan or dextran, myeloperoxidase activity, neutrophil migration induced by carrageenan, IL-1β concentration, and increase on IL-10 level in the peritoneal fluid of animals. Our results suggest that anti-oxidative and anti-inflammatory present in the brown seaweed S. schroederi may be related to the presence of flavonoids.

Keywords

Flavonoid content TOAC Paw edema Peritonitis 

Notes

Acknowledgments

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Sérvio Quesado Júnior
    • 1
  • Victor Hugo Azevedo Carneiro
    • 1
  • Thais Pontes Carvalho Fontenelle
    • 1
  • Luciano de Sousa Chaves
    • 1
  • Jacilane Ximenes Mesquita
    • 1
  • Tarcísio Vieira de Brito
    • 2
  • Rafael Siva Prudêncio
    • 2
  • Jefferson Soares de Oliveira
    • 2
  • Jand-Venes Rolim Medeiros
    • 2
  • Karoline Saboia Aragão
    • 3
  • Ronaldo de Albuquerque Ribeiro
    • 3
  • André Luiz dos Reis Barbosa
    • 2
  • Ana Lúcia Ponte Freitas
    • 1
  1. 1.Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular BiologyFederal University of CearáFortalezaBrazil
  2. 2.Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC)Federal University of PiauíParnaíbaBrazil
  3. 3.Laboratory of Pharmacology of Inflammation and Cancer, Department of Physiology and PharmacologyFederal University of CearáFortalezaBrazil

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