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Effects of spindle inhibitors and phytoregulators on the micropropagation of Kappaphycus alvarezii (Rhodophyta, Gigartinales)

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Abstract

Spindle inhibitors and phytoregulators are substances largely used to improve the growth of plants with commercial interest. This study analyzes the effects of colchicine (Ch) and oryzalin (Oz), two spindle inhibitors, on the survival and growth rates of Kappaphycus alvarezii micropropagules when combined in cultures with such phytoregulators as indole-3-acetic acid (IAA), kinetin (K), and spermine (S), aiming to improve mass cultivation under laboratory conditions. Explants were first cultured in treatments of colchicine and oryzalin, separately or in combination, for 2 weeks. Afterwards, they were cultured in sterilized seawater enriched with 50 % von Stosch solution. All explants produced upright axes from the cut or lateral region. Treatment with 1.0 mg L−1 oryzalin significantly increased the formation of upright axes (4.89 ± 1.60 upright axes explant−1) compared to control (1.33 ± 0.16 upright axes explant−1). Explants treated with oryzalin alone or in combination with IAA or kinetin showed a significantly higher production of upright axes, while those treated with oryzalin in combination with spermine or other phytoregulators (IAA and K; IAA and S; K and S; IAA, K, and S) showed a significant increase of growth rates, ranging 3.47 ± 0.27 % day−1in control to 4.98 ± 0.47 % day−1in the IAA:S + Oz treatment. Results showed that oryzalin in combination with phytoregulators, such as IAA, spermine, or kinetin, affects micropropagule production and growth, thereby improving the mass cultivation of K. alvarezii but without affecting the survival rates of explants.

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Acknowledgments

This study received financial support from the Coordenação de Aperfeiçoamento de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Botany Department, Federal University of Santa Catarina (UFSC), Florianópolis, 88040-970, Brazil

    Filipe A. S. Neves & Leila Hayashi

  2. Cellular Biology, Embryology and Genetics Department, UFSC, Florianópolis, 88040-970, Brazil

    Carmen Simioni & Zenilda L. Bouzon

  3. Aquaculture Department, UFSC, Rodovia Admar Gonzaga, 1346, Itacorubi, Florianópolis, 88034-001, Brazil

    Leila Hayashi

Authors
  1. Filipe A. S. Neves
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  2. Carmen Simioni
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  3. Zenilda L. Bouzon
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  4. Leila Hayashi
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Correspondence to Filipe A. S. Neves.

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Neves, F.A.S., Simioni, C., Bouzon, Z.L. et al. Effects of spindle inhibitors and phytoregulators on the micropropagation of Kappaphycus alvarezii (Rhodophyta, Gigartinales). J Appl Phycol 27, 437–445 (2015). https://doi.org/10.1007/s10811-014-0309-3

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  • DOI: https://doi.org/10.1007/s10811-014-0309-3

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