A novel actinomycete Streptomyces aurantiogriseus with algicidal activity against the toxic cyanobacterium Microcystis aeruginosa
A novel actinomycete strain (PK1) was isolated from soil in Khon Kaen Province, Thailand, and was capable of inhibiting the cyanobacterium Microcystis aeruginosa. The isolate PK1 was identified as Streptomyces aurantiogriseus based on an analysis of biochemical and morphological characteristics and 16S rDNA sequence. The algicidal activity of PK1 against M. aeruginosa depended on the growth phase of PK1, but not on the cyanobacterial growth phase. Stationary growth phase cultures of the strain PK1 exhibited the highest anti-Microcystis activity when co-cultivated with M. aeruginosa. Complete growth inhibition was observed after 8 days of co-cultivation in liquid culture medium. The algicidal compounds were extracted from PK1 with ethyl acetate and then purified by silica gel column chromatography. These partially purified compounds demonstrated algicidal activity against M. aeruginosa, suggesting that the strain PK1 provides a potential source of extracellular compounds for the control of M. aeruginosa bloom. This is the first report of anti-cyanobacterial activity from the soil actinomycete S. aurantiogriseus.
KeywordsStreptomyces aurantiogriseus Microcystis aeruginosa anti-Microcystis actinomycetes
The authors thank the Plant Genetics Conservation Project under The Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn for the permission to conduct the research. We would also like to thank Assoc. Prof. Dr. Somdej Kanokmedhakul for the assistance with the fractionation procedure and for the useful discussions.
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