A novel actinomycete Streptomyces aurantiogriseus with algicidal activity against the toxic cyanobacterium Microcystis aeruginosa
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A novel actinomycete strain (PK1) was isolated from soil in Khon Kaen Province, Thailand, and was capable of inhibiting the cyanobacterium Microcystis aeruginosa. The isolate PK1 was identified as Streptomyces aurantiogriseus based on an analysis of biochemical and morphological characteristics and 16S rDNA sequence. The algicidal activity of PK1 against M. aeruginosa depended on the growth phase of PK1, but not on the cyanobacterial growth phase. Stationary growth phase cultures of the strain PK1 exhibited the highest anti-Microcystis activity when co-cultivated with M. aeruginosa. Complete growth inhibition was observed after 8 days of co-cultivation in liquid culture medium. The algicidal compounds were extracted from PK1 with ethyl acetate and then purified by silica gel column chromatography. These partially purified compounds demonstrated algicidal activity against M. aeruginosa, suggesting that the strain PK1 provides a potential source of extracellular compounds for the control of M. aeruginosa bloom. This is the first report of anti-cyanobacterial activity from the soil actinomycete S. aurantiogriseus.
KeywordsStreptomyces aurantiogriseus Microcystis aeruginosa anti-Microcystis actinomycetes
The authors thank the Plant Genetics Conservation Project under The Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn for the permission to conduct the research. We would also like to thank Assoc. Prof. Dr. Somdej Kanokmedhakul for the assistance with the fractionation procedure and for the useful discussions.
- Ara I, Bukhari NA, Aref NM, Shinwari MMA, Bakir MA (2012) Antiviral activities of Streptomycetes against tobacco mosaic virus (TMV) in Datura plant: evaluation of different organic compounds in their metabolites. Afr J Biotechnol 11:2130–2138Google Scholar
- Atta HM, Ahmad MS (2009) Antimycin-A antibiotic biosynthesis produced by Streptomyces sp. AZ-AR-262: taxonomy, fermentation, purification and biological activities. Aust J Basic Appl Sci 3:126–135Google Scholar
- DeFrank J, Putnam AR (1985) Screening procedures to identify soil-borne Actinomycetes that can produce herbicidal compounds. Weed Sci 33:271–274Google Scholar
- El-Khawagh MA, Hamadah KS, El-Sheikh TM (2011) The insecticidal activity of Actinomycete metabolites, against the mosquito Culex pipiens. Egypt Acad J Biol Sci 4:103–113Google Scholar
- Holt JG, Kreig NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey's manual of determinative bacteriology, 9th edn. Lippincott, BaltimoreGoogle Scholar
- Hoshaw R, Rosowski JR (1973) Method for microscopic algae. In: Stein JR (ed) Handbook of phycological methods, culture methods and growth measurements. Cambridge University Press, London, pp 53–56Google Scholar
- McGuire MJ, Jones RM, Means EG, Izaguirre G, Preston AE (1984) Controlling attached blue-green algae with copper sulfate. J Am Water Works Assoc 76:60–65Google Scholar
- Ruiz MLV, Silva PG, Laciar AL (2009) Comparison of microplate, agar drop and well diffusion plate methods for evaluating hemolytic activity of Listeria monocytogenes. Afr J Microbiol Res 3:319–324Google Scholar
- Sivonen K, Jones G (1999) Cyanobacterial toxins. In: Chorus I, Bartran J (eds) Toxic cyanobacteria in water: a guide to their public health consequences, monitoring and management. E&FN Spon, London, pp 41–111Google Scholar
- Williams ST, Goodfelow M, Alderson G (1989) Genus Streptomyces Waksman and Henrici 1943, 339AL. In: William ST, Sharpe ME (eds) Bergey's manual of systematic bacteriology, vol 4. Lippincott, Baltimore, pp 2452–2492Google Scholar