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Journal of Applied Phycology

, Volume 26, Issue 3, pp 1511–1518 | Cite as

Purification of biosilica from living diatoms by a two-step acid cleaning and baking method

  • Wenkai Jiang
  • Songping Luo
  • Pengwei Liu
  • Xiangyun Deng
  • Yani Jing
  • Chengying Bai
  • Jianbao Li
Article

Abstract

Due to their sustainability, intact cell walls, availability of pure cultures, and others, living diatoms show a lot of promise for the application in various fields in particular for micro/nano-devices. In order to purify the biosilica structures of diatoms called frustules, a two-step acid cleaning and baking method was employed. By this path, organic matter and inorganic impurities can be removed very effectively. In addition, the highest quality of frustules was achieved when the samples were cleaned in an excess of boiling 10~15 % HCl and subsequently heated to 600 °C at a heating rate of 3 °C min−1 for 6 h. In our operation, the native frustule morphology was maintained completely, and dry frustules with more than 90 % SiO2 in weight can be obtained, and furthermore, the surface area of them reached a good value of 48.47 m2 g−1.

Keywords

Living diatoms Frustules Biosilica Purification Baking Acid cleaning 

Notes

Acknowledgments

The work was supported by the 863 project under Grant No.2012AA03A610, Hainan University Research Fund and “211” innovation platform of Hainan University.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Wenkai Jiang
    • 1
  • Songping Luo
    • 1
    • 3
  • Pengwei Liu
    • 1
  • Xiangyun Deng
    • 1
    • 2
  • Yani Jing
    • 1
  • Chengying Bai
    • 1
  • Jianbao Li
    • 1
    • 3
  1. 1.Key Laboratory of Ministry of Education for Advanced Material in Tropical Island ResourcesHainan UniversityHaikouPeople’s Republic of China
  2. 2.College of Physics and Electronic InformationTianjin Normal UniversityTianjinPeople’s Republic of China
  3. 3.State Key Laboratory of New Ceramics & Fine Processing, Department of Material Science and EngineeringTsinghua UniversityBeijingPeople’s Republic of China

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