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Journal of Applied Phycology

, Volume 26, Issue 1, pp 417–420 | Cite as

Microsatellite markers for the palaeo-temperature indicator Pentapharsodinium dalei (Dinophyceae)

  • Nina Lundholm
  • Lene Rostgaard Nielsen
  • Sofia Ribeiro
  • Marianne Ellegaard
Article

Abstract

Pentapharsodinium dalei is a widely distributed cold-water dinoflagellate, which is used in palaeoecology as an indicator of relatively warmer conditions in polar and sub-polar regions. This species has been proposed to be one of the first indicators of global warming at high latitudes. We developed the first microsatellite markers for P. dalei to facilitate the study of spatial and temporal population genetic changes. Single cysts were isolated from surface sediments in Koljö Fjord, Sweden. After cyst germination, single vegetative cells were isolated for establishing monoclonal cultures. Six dinucleotide polymorphic microsatellite markers were developed as multiplex polymerase chain reactions and were genotyped in 32 strains. The number of alleles per locus varied between 4 and 12, and the estimated gene diversity varied from 0.588 to 0.891. The haploid state of the vegetative cells was confirmed. The six selected microsatellites will be useful to explore population dynamics in P. dalei from contemporary planktonic and revived benthic samples to enable, for example, detailed studies into the evolutionary consequences of anthropogenic and climate-driven habitat changes.

Keywords

Dinoflagellate Global change Microsatellites Multiplex PCR Pentapharsodinium dalei Population genetics Sediment Temperature 

Notes

Acknowledgments

This study was part of the Danish Research Council project 2111-04-0011. SR holds a postdoctoral grant from the Carlsberg Foundation, Denmark (no. 2011_01_0337).

References

  1. Armour JA, Neumann R, Gobert S, Jeffreys AJ (1994) Isolation of human simple repeat loci by hybridization selection. Human Mol Gen 3:599–605CrossRefGoogle Scholar
  2. Dale B (1996) Dinoflagellate cyst ecology: modelling and geological applications. In: Jansonius J, McGregor DC (eds) Palynology: principles and applications. The American Association of Stratigraphic Palynologists Foundation, Salt Lake City, pp 1249–1276Google Scholar
  3. Dale B (2001) The sedimentary record of dinoflagellate cysts: looking back into the future of phytoplankton blooms. Sci Mar 65:257–272CrossRefGoogle Scholar
  4. Doyle JJ, Doyle JL (1990) A rapid total DNA preparation procedure for fresh plant tissue. Focus 12:13–15Google Scholar
  5. Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinformat Online 1:47–50Google Scholar
  6. Gautschi B, Tenzer I, Müller JP, Schmid B (2000a) Isolation and characterization of microsatellite loci in the bearded vulture (Gypaetus barbatus) and cross-amplification in three Old World vulture species. Mol Ecol 9:2193–2195PubMedCrossRefGoogle Scholar
  7. Gautschi B, Widmer A, Koella J (2000b) Isolation and characterization of microsatellite loci in the dice snake (Natrix tessellata). Mol Ecol 9:2191–2193PubMedGoogle Scholar
  8. Hackett JD, Anderson DM, Erdner DL, Bhattacharya D (2004) Dinoflagellates: a remarkable evolutionary experiment. Am J Bot 91:1523–1534PubMedCrossRefGoogle Scholar
  9. Harland R, Nordberg K, Filipsson HL (2004) A high-resolution dinoflagellate cyst record from latest Holocene sediments in Koljö Fjord, Sweden. Rev Palaeobot Palynol 128:119–141CrossRefGoogle Scholar
  10. Larsen NH, Moestrup Ø, Pedersen PM (1994) Catalogue 1994. Scandinavian Culture Centre for Algae & Protozoa. Department of Phycology. Botanical Institute. University of Copenhagen. http://www.sccap.dk/media/marine/1.asp
  11. Lundholm N, Daugbjerg N, Moestrup Ø (2002) Phylogeny of the Bacillariaceae with emphasis on the genus Pseudo-nitzschia (Bacillariophyceae) based on partial LSU rDNA. Eur J Phycol 37:115–134CrossRefGoogle Scholar
  12. Lundholm N, Ribeiro S, Andersen TJ, Kock T, Godhe A, Ekelund F, Ellegaard M (2011) Buried alive—germination of up to a century-old marine protist resting stages. Phycologia 6:629–640CrossRefGoogle Scholar
  13. Masseret E, Grzebyk D, Nagai S, Genovesi B, Lasserre B, Laabir M, Collos Y, Vaquer A, Berrebi P (2009) Unexpected genetic diversity among and within populations of the toxic dinoflagellate Alexandrium catenella as revealed by nuclear microsatellite markers. Appl Environ Microbiol 75:2037–2045PubMedCentralPubMedCrossRefGoogle Scholar
  14. McQuoid MR, Godhe A, Nordberg K (2002) Viability of phytoplankton resting stages in the sediments of a coastal Swedish fjord. Eur J Phycol 37:1–11CrossRefGoogle Scholar
  15. Nei M (1987) Molecular evolutionary genetics, 1st edn. Columbia University, New YorkGoogle Scholar
  16. Peakall R, Smouse PE (2006) GenAlEx 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295CrossRefGoogle Scholar
  17. Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update. Bioinformatics 28:2537–2539PubMedCentralPubMedCrossRefGoogle Scholar
  18. Penaud A, Eynaud F, Sanchez-Goñi M, Malaizé B, Turon JL, Rossignol L (2011) Contrasting sea-surface responses between the western Mediterranean Sea and eastern subtropical latitudes of the North Atlantic during abrupt climatic events of MIS 3. Mar Micropaleont 80:1–17CrossRefGoogle Scholar
  19. Pfiester LA, Anderson DM (1987) Dinoflagellate reproduction. In: Taylor FJR (ed) The biology of dinoflagellates. Blackwell, Oxford, pp 611–648Google Scholar
  20. Ribeiro S, Moros M, Ellegaard M, Kuipers A (2011a) Climate variability in West Greenland during the past 1500 years: evidence from a high-resolution marine palynological record from Disko Bay. Boreas 41:68–83CrossRefGoogle Scholar
  21. Ribeiro S, Berge T, Lundholm N, Andersen TJ, Abrantes F, Ellegaard M (2011b) Phytoplankton growth after a century of dormancy illuminates past resilience to catastrophic darkness. Nat Commun. doi: 10.1038/ncomms1314 PubMedCentralPubMedGoogle Scholar
  22. Rochon A, Vernal A, Turon J, Matthießen J, Head M (1999) Distribution of recent dinoflagellate cysts in surface sediments from the North Atlantic Ocean and adjacent seas in relation to sea-surface parameters. Am Ass Stratigr Palynol Contrib Ser 35:1–146Google Scholar
  23. Santos SR, Coffroth MA (2003) Molecular genetic evidence that dinoflagellates belonging to the genus Symbiodinium Freudenthal are haploid. Biol Bull 204:10–20PubMedCrossRefGoogle Scholar
  24. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234PubMedCrossRefGoogle Scholar
  25. Spector DL (1984) Dinoflagellate nuclei. In: Spector DL (ed) Dinoflagellates. Academic, Orlando, pp 107–147CrossRefGoogle Scholar
  26. Sperisen C, Gugerli F, Bucher U, Mátyás G (2000) Comparison of two rapid DNA extraction protocols for gymnosperm for application in population genetic and phylogenetic studies. Forest Gen 7:133–136Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Nina Lundholm
    • 1
  • Lene Rostgaard Nielsen
    • 2
  • Sofia Ribeiro
    • 3
  • Marianne Ellegaard
    • 4
  1. 1.The Natural History Museum of DenmarkUniversity of CopenhagenCopenhagen KDenmark
  2. 2.Department of Geosciences and Natural Resource ManagementUniversity of CopenhagenFrederiksberg CDenmark
  3. 3.Marine Geology and Glaciology DepartmentGeological Survey of Denmark and Greenland (GEUS)Copenhagen KDenmark
  4. 4.Department of BiologyUniversity of CopenhagenCopenhagen KDenmark

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